表皮生长因子对家兔角膜内皮损伤修复作用的实验研究  

Effects of epidermal growth factor on corneal endothelial cell wound healing in rabbits

作　　者：杨蕊[1] 郭绒霞[1] 孙乃学[1] 王美纳[1] 

机构地区：[1]西安医科大学第二临床医学院眼科

出　　处：《眼科研究》1999年第4期279-282,共4页Chinese Ophthalmic Research

摘　　要：目的研究表皮生长因子（ＥＧＦ）对家兔角膜内皮损伤的修复作用。方法将溶于透明质酸钠（ＮａＨＡ）的ＥＧＦ注入角膜内皮已损伤的兔眼前房，对照眼只注入ＮａＨＡ。结果１０μｇ和５μｇ的ＥＧＦ均可使角膜内皮缺损面积显著缩小（Ｐ＜０．０５），并可使再生的角膜中央内皮细胞密度（ＥＣＤ）、六边形细胞出现率显著上升（Ｐ＜０．０５）及细胞面积变异系数明显减小（Ｐ＜０．０５）。结论１０μｇ和５μｇ的ＥＧＦ均可促进家兔角膜内皮损伤的愈合及内皮细胞大小和形态的早期恢复。Objective To study the effects of epidermal growth factor(EGF) on rabbit corneal endothelial cell wound healing in vivo. Methods EGF was administered to the anterior chamber of rabbits in sodium hyaluronate(NaHA) after an endothelial cells cryoinjury.Eyes of control group received NaHA alone. Results EGF(10μg,5μg)can significantly decrease the area of endothelial cell wound compared with that of the control eyes ( P <0.05).EGF(10μg,5μg)can significantly increase the regeneration of endothlial cell density(ECD),and the percentage of hexagonal cells( P <0.05).The coefficient of variation of the cell size was decreased compared with that of the control eyes ( P <0.05). Conclusion The result indicates that EGF(10μg,5μg)can enhance the wound healing of endothelial cell and stimulate an early restoration of cell size and shape.

关 键 词：表皮生长因子 角膜内皮损伤 兔 

分 类 号：R772.2[医药卫生—眼科]