军牧1号白猪肉质主效基因的多态性检测分析  

Detection polymorphism of the meat major gene of the Junmu No.1 white pig

作　　者：马焕[1] 赵伟[1] 王大力[1] 邢沈阳[1] 孙博兴[1] 赵志辉[1] 黄大鹏[2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国兽医学报》2011年第1期129-132,共4页Chinese Journal of Veterinary Science

基　　金：国家星火计划重点资助项目(2008GA661001);吉林省科技支撑资助项目(200800208)

摘　　要：猪的肉质属于数量性状,受多个主效基因和候选基因的控制。为检测军牧1号白猪肉质主效基因多态性,本试验采用RFLP技术检测了400头军牧1号白猪氟烷基因(RYR1)、心脏脂肪酸结合蛋白基因(H-FABP)和酸肉基因(RN)3个重要肉质基因的多态性。群体遗传结构分析表明,军牧1号白猪群体中RYR1基因的N基因频率为0.908 3,n基因频率为0.091 7;心脏脂肪酸结合蛋白基因的5′区的HinfⅠ位点的H基因频率为0.688 8,h基因频率为0.311 2;H-FABP基因内含子-2HaeⅢ位点D基因频率为0.478 8,d基因频率为0.521 2;H-FABP基因内含子-2MSPⅠ位点不存在多态性;RN基因也不存在多态性。Pork quality is a quantity trait,which is controlled by several major genes and many candicate genes.In order to detect the polymorphism of meat quality major gene of Junmu No.1 white pig,the halothane gene（RYR1）,Rendement napole（RN）,and heart fatty acid binding protein gene（H-FABP） were checked by PCR-RFLP among the 400 Junmu No.1 white pig.The result indicated that,the frequency of N type RYR1 gene was 0.908 3,n was 0.091 7;in H-FABP gene 5 ＇region HinfⅠ,the frequency of H was 0.688 8,h was 0.311 2;in H-FABP intron 2 of the Hae Ⅲ locus,the frequency of D was 0.478 8,d was 0.521 2;no polymorphism evidence was found in intron 2 of H-FABP MSPⅠ sites and Rendement Napole gene.

关 键 词：军牧1号白猪 RYR1基因 RN基因 H-FABP基因 遗传多态性 

分 类 号：S828.2[农业科学—畜牧学]