结直肠腺癌中候选干细胞标记基因Musashi-1和β1-integrin的表达  

Expression of putative stem cell genes Musashi-Ⅰ and Ⅲ-integrin in human colorectal adenocarcinomas

作　　者：吴云华[1] 江从庆[1] 钱群[1] 刘志苏[1] 夏冰 

机构地区：[1]武汉大学中南医院结直肠肛门外科结直肠肛门疾病研究中心湖北省肠病医学临床研究中心,430071 [2]湖北省肠病医学临床研究中心

出　　处：《中华实验外科杂志》2011年第1期37-38,161,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察候选干细胞标记基因Musashi-1、β1-integrin在结直肠腺瘤及结直肠腺癌组织中的表达。方法应用免疫组织化学链霉生物素-过氧化物酶法（SP法），检测结直肠癌组织芯片（8例正常结直肠黏膜，8例腺瘤，69例腺癌，其中8例为黏液腺癌组织）中Musashi-1和β1-integrin蛋白表达，分析其与临床病理特征的关系。结果结直肠腺癌组织中Musashi-1阳性表达率为66．7％（46／69），腺瘤为50．0％（4／8）。结直肠腺瘤和腺癌中β1-integrin阳性表达率分别为37．5％（3／8）和59．2％（41／69）（P〈0．05）。腺癌组β1-integrin阳性表达率显著高于腺瘤组（P〈0．05）。TNMⅢ期腺癌组Musashi-1和β1-integrin蛋白表达显著高于TNMⅠ-Ⅱ期组（P〈0．05）。相关分析显示，Musashi-1与β1-integrin呈正相关（0=0．631，P〈0．01）。结论候选干细胞标记基因Musashi-1、β1-integrin可能在结直肠癌发生、进展中起重要作用。Objective To investigate the expression of two putative stem cell genes Musashi-1 and β1-integrin in human colorectal carcinomas. Methods Musashi-1 and β1-integrin immunoreactivity was studied immunohistochemically in tissue microarray-based samples containing 69 cases of colorectal adenocarcinomas, 8 cases of normal mucosa, and 8 cases of adenomas. Results Musashi-1 protein expression rate was 66.7% （46/69） in colorectal adenocarcinomas. The positive rate of β1-integrin protein was 59. 2% （41/69） in adenocareinomas, higher than in the colorectal adenomas tissues （37.5% , 3/8, P 〈 0.05 ）. The expression levels of Musashi-1 and β1-integrin protein were significantly higher in samples of TNM stage HI than in those of TNM stage Ⅰ -Ⅱ （P 〈 0. 05 ）. Musashi-1 expression was closely correlated with β1-integrin （rs = 0. 631 ,P 〈 0. 01 ）. Conclusion Putative intestinal stem cell genes Musashi-1 and β1-integrin may be involved in human colorectal tumor carcinogenesis and progression.

关 键 词：结直肠癌 干细胞 

分 类 号：R735.3[医药卫生—肿瘤]