颅内动脉瘤形成与辅助性T细胞17免疫应答的关系  

T helper 17 in the formation of intracranial aneurysm

作　　者：李美华[1] 凌峻[3] 熊秋云[2] 聂艳良[1] 梅金红[2] 

机构地区：[1]南昌大学第一附属医院神经外科,330006 [2]南昌大学第一附属医院病理科,330006 [3]赣南医学院第一附属医院

出　　处：《中华实验外科杂志》2011年第1期92-94,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30960397）;江西省自然科学基金资助项目（2009GZY0200）;江西省卫生厅资助项目（20091063）.

摘　　要：目的观察辅助性T细胞17（Th17）免疫应答是否参与颅内动脉瘤形成过程，检测细胞因子白细胞介素17（IL-17）及其Th17免疫应答特异性标志物一特异转录因子孤束核受体（ROR-γt）在大鼠和人颅内动脉瘤壁中的表达。方法建立肾性高血压诱导的Sprague—Dawley（SD）大鼠颅内动脉瘤模型，获取8个sD大鼠颅内动脉瘤。另取15例人颅内动脉瘤组织标本，用免疫组织化学方法检测IL-17、RORγt在大鼠脑动脉瘤壁中的表达，以及人脑动脉瘤壁IL-17、RORγt、白细胞共同抗原（LCA）和CD4的表达。结果SD大鼠颅内动脉瘤壁上IL-17和RORγt有明显表达，且IL-17和RORγt表达呈正相关（r=0．917，P〈0．01），而正常对照SD大鼠脑动脉血管壁上无IL-17和RORγt的表达。IL-17和RORγt在人颅内动脉瘤壁也明显表达且呈正相关（r=0．903，P〈0．01），而正常对照颞浅动脉壁上无IL-17和RORγt的表达。同时，在人脑动脉瘤壁中IL-17和RORγt阳性表达的部位LCA和CD4也呈阳性。结论在大鼠和人的颅内动脉瘤壁均检测到IL-17和RORγt表达，表明Th17应答可能参与了颅内动脉瘤的形成。Objective To detect the level of interleukln 17 （IL-17） and transcription factor of orphan nuclear hormone receptor-γt （RORγt） in the intracranial aneurysm of humans and rats, and confirm whether T helper 17 （ Th17 ） takes part in the formation of intracranial aneurysm. Methods The models of intracranial aneurysm induced by nephrotic hypertension in Sprague-Dawley （ SD ） rats were established. Eight aneurysmal changes were found in the junction of the anterior cerebral （ACA） and the olfactort artery （OA） of rats, and immunohistochemistry was used to detect the expression level of IL-17 and RORTt, as well as 15 patients with intracranial aneurysms. Results Both IL-17 and RORγt were obviously expressed in the brain of rats with intracranial aneurysms as compared with the opposite control group. The percentage of IL-17 positive cells was associated with the percentage of RORγt positive cells （r = 0. 917 ,P 〈 0. 01 ）. No intraeranial aneurysm was induced in control group. Both IL-17 and RORγt in patients with intracranial aneurysms were obviously expressed, as well as LCA and CD4. The percentage of IL-17 positive cells was associated with the percentage of RORγt positive cells （r =0. 903 ,P 〈0. 01 ）. There was no expression of IL-17 and RORγt in the superficial temporal artery of normal controls. Conclusion Obvious expression of IL-17 and RORγt in the intracranial aneurysm of humans and rats may imply that Th17 response involves in the formation of intracranial aneurysm.

关 键 词：颅内动脉瘤 T细胞 白细胞介素-17 

分 类 号：R743[医药卫生—神经病学与精神病学]