转录因子Sp1调节儿童横纹肌肉瘤成纤维细胞生长因子基因表达的机制  

Mechanism of regulation of FGFR4 gene expression by transcription factor Sp1 in human infant rhabdomyosarcomas

作　　者：于顺江[1] 陈力[1] 陈怡东[1] 王京[2] 

机构地区：[1]北京世纪坛医院(北京大学第九临床学院)肿瘤放疗科,100038 [2]北京世纪坛医院(北京大学第九临床学院)病理科,100038

出　　处：《中华实验外科杂志》2011年第1期136-138,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨转录因子Sp1调节儿童横纹肌肉瘤成纤维细胞生长因子受体4（FGFR4）基因表达的作用及其调节的分子机制。方法应用细胞及分子生物学技术如细胞转染、RNA和蛋白质印迹等方法检测2种不同横纹肌肉瘤细胞株中的FGFR4mRNA和蛋白的表达，观察Sp1对FG—FR4表达的调节作用，探讨Sp1调节FGFR4基因表达的机制。结果Sp1在儿童横纹肌肉瘤中可高度表达，Sp1与FGFR4启动区结合并上调了FGFR4的表达。Sp1的这一调节作用不受FGFR4启动区甲基化作用的影响。结论Sp1促进FGFR4在儿童横纹肌肉瘤细胞中的表达，该作用可能是通过Sp1与FGFR4启动区的顺式元件直接结合而实现的。Objective To investigate the role of transcription factor Spl in the fibroblast growth factor receptor 4 （FGFR4） expression regulation and to obtain insight into the regulatory mechanisms governing FGFR4 gene regulation and overexpression in human infant rhabdomyosarcomas. Methods By using a series of cellular and molecular biology methods like transfection, Northern blotting, Western blotting etc. , the mRNA and protein expression of FGFR4 was detected. The regulatory effects of Spl on the FG- FR4 expression in the human rhabdomyosarcoma-derived cells were analyzed. Results Spl could be express highly in the human rhabdomyosarcoma-derived cells, and its binding to FGFR4 promoter could upregulate the FGFR4 expression, which was influenced by the methylation of FGFR4 promoter. Condusion Spl induces the expression of FGFR4 in human infant rhabdomyosareomas by directly binding of Spl with DNA cis elements in FGFR4 gene promoter region.

关 键 词：转录因子 基因表达 横纹肌肉瘤 

分 类 号：R738[医药卫生—肿瘤]