30日龄Fmr1基因敲除小鼠的自主活动观察  

Behavioural comparision on Fmr1 knockout mice at 30 days age in spontaneous activity test

作　　者：张伟雯[1] 黄越玲[2] 刘国彬[3] 沈岩松[3] 孙卫文[3] 李敏雄[3] 戴丽军[1] 陈盛强[3] 

机构地区：[1]广州医学院第三附属医院,510150 [2]广州医学院第三附属医院动物实验中心,510150 [3]广州医学院第二附属医院

出　　处：《国际医药卫生导报》2011年第1期1-4,共4页International Medicine and Health Guidance News

基　　金：广州市医药卫生科技项目（2009-YB-167）;广东省自然科学基金项目（815101700100005）;广东省科技计划项目（2005B60302004、2008B030301371、2009B030801368）;广州市中医药中西医结合科研立项项目（2008A52）

摘　　要：目的 对30日龄的Fmr1基因敲除小鼠的自主活动进行观察.方法 采用30日龄的KO鼠和WT鼠分别连续进行2天的自主活动实验,根据所获得的数据进行多因素方差分析处理.结果 通过第1天的学习与第2天的记忆再现,在自主活动实验中,与WT鼠相比,KO鼠在自主活动实验中的活动和站立次数均增多,具有统计学意义（P＜0.05）,粪便数相比无明显差异.结论 30日龄Fmr1基因敲除小鼠的自主活动异常,运动性和兴奋性较野生型小鼠增高.Objective To compare the behavioural differences at 30 days age in spontaneous activity test. Methods Fmr1 knockout mice were identified using the PCR technica. and spontaneous activity test was used in the study .The data was analyzed with Multifactor Variance Analysis. Results Activities in their own experiments, as compared with WT mice, KO mice in the experiment of self-activity increase in the number of activities and reduction in the number of standing, with statistical significance （ P〈0.05 ）, but there were no significant differences on manure behaviour between two groups. Conclusion Fmr1 knockout animals exhibited higher locomotor activity in the spontaneous activity test at 30 days age.

关 键 词：自主活动 FMR1基因敲除小鼠 

分 类 号：R[医药卫生]