肺癌患者血清DKK1、CYFRA21-1、SCC、NSE水平及临床意义  

DKK1,CYFRA21-1,SCC,NSE Serum Level in Patients with Lung Cancer and its Clinical Significance

作　　者：孙红岗[1,2] 董学君[2] 李志国[1] 杨超[1] 卢韬[1] 

机构地区：[1]温州医学院,325035 [2]浙江省绍兴市人民医院分子医学中心,312000

出　　处：《医学研究杂志》2010年第12期67-70,共4页Journal of Medical Research

基　　金：浙江省医药卫生科学研究基金资助项目(2009A208);绍兴市科技计划项目(2009A23005)

摘　　要：目的探讨肺癌患者血清DKK1、CYFRA21-1、SCC、NSE水平及其临床应用价值。方法病理确诊的肺部肿瘤患者80例(肺癌70例,肺部良性肿块病例10例),健康体检人员10例。用ELISA试剂盒检测血清标本中DKK1浓度,免疫化学发光法测血清CYFRA21-1、SCC、NSE浓度。计数资料采用2χ检验,组间均数比较采用t检验,ROC曲线评价各指标联合应用的效果。结果与正常对照组比较,非小细胞肺癌、小细胞癌中DKK1浓度升高,具有统计学意义(P<0.05),而肺部良性肿块组与正常对照组间无意义(P>0.05)。肺癌转移组血清DKK1浓度明显高于未转移组,二者有显著意义(P<0.05)。肺癌患者术后血清DKK1浓度比术前下降明显(P<0.05)。血清DKK1与CYFRA21-1、SCC、NSE联合诊断,可明显提高非小细胞肺癌诊断的敏感性与特异性。结论 DKK1、CYFRA21-1、SCC、NSE联合诊断对肺癌诊断的价值更高;血清DKK1是肺癌诊断、转移及治疗效果评价的有效指标。Objective To detect DKK1, CYFRA21 - 1, SCC, NSE expression in the patients with lung cancer and to evaluate their clinical value. Methods The study included 70 lung cancer patients, 10 benign lung tumor patients and 10 controls. All of them were confirmed by pathology. ELISA method was applied to detect serum DKK1 concentration, and ARCHITECT i2000SR immunoassay to de- tect serum CYFRA21 - 1, SCC, NSE concentration. Numeration data was analyzed hyx2 test, and groups differences were compared by t test, and these indicators were evaluated by ROC curve. Results DKK1 concentrations in serum of non - small cell lung cancer and small cell lung cancer were significantly higher than those of the normal controls （P 〈 0.05）. And the difference of DKK1 concentration between in serum of benign lung tumor and the controls was not statistically significant （P 〉 O. 05 ）. Serum DKK1 concentration in the me- tastasis group was significantly higher than that in premetastatic group （ P 〉 0.05）. Serum DKK1 concentration in surgery patients with lung cancer was significantly higher than that in the preoperative group （P 〉 O. 05）. While combined detection of serum DKK1 and CY- FRA21 - 1, SCC, NSE could significantly improve the sensitivity and specificity of diagnosis of lung cancer. Conclusion Combined use of DKK1, CYFRA21 - 1, SCC, NSE for the diagnosis of lung cancer has even greater value. Serum DKK1 can be used for diagnosis of lung cancer, metastasis and monitoring treatment.

关 键 词：肺癌 DKK1 联合诊断 预后 

分 类 号：R734.2[医药卫生—肿瘤]