GRIM-19的表达对人宫颈癌细胞移植瘤生长的影响  

Effects of GRIM-19 on Growth of Cervical Cancer Cell Xenograft Tumor

作　　者：程勇[1] 卫莹[2] 李敏[2] 周颖[2] 冯定庆[2] 肖卫华[3] 凌斌[2] 

机构地区：[1]安徽医科大学附属省立医院肿瘤放疗科,合肥230001 [2]安徽医科大学附属省立医院妇产科分子实验室安徽省分子医学重点实验室,合肥230001 [3]中国科技大学免疫所

出　　处：《肿瘤防治研究》2010年第12期1343-1345,共3页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(81072127;81001168);安徽省自然科学基金资助项目(090413117);安徽省高等学校省级自然科学研究资助项目(KJ2010B375)

摘　　要：目的探讨GRIM-19对宫颈癌细胞株HeLa增殖行为的影响及其机制。方法采用RT-PCR和Western blot检测基因的mRNA和蛋白水平表达情况。将HeLa/control、HeLa/GRIM-19接种到裸鼠皮下检测荷瘤情况,利用免疫组织化学法检测Ki-67的表达。结果 HeLa/GRIM-19细胞接种肿瘤生长速度明显减慢(P<0.01)。移植瘤的RT-PCR结果:GRIM-19表达升高、而其下游基因STAT3、Cyclin B1、Bcl-L2的表达降低;蛋白检测发现STAT3、p-STAT3、suvivin、Ki-67基因的表达均下降,GRIM-19表达升高。结论 GRIM-19的表达可以抑制宫颈癌细胞的增殖,可能是宫颈癌治疗的新靶点。Objective To explore the effects of GRIM-19 expression on the growth of cervical cancer in vivo and its mechanisms.Methods GRIM-19 gene expression was investigated at mRNA and protein levels by RT-PCR and Western blot.Tumor growth of the null mice was analyzed after injection HeLa/control,HeLa/GRIM-19 into the skin.The protein level of Ki-67 was inspected with immumohistochemistry methods.Results The growth of HeLa/GRIM-19 implanted tumor slowed down significantly（P0.01）.The expression of GRIM-19 mRNA was up-regulated,while STAT3,cyclin B1,Bcl-L2 mRNA were decreased in tumors bearing HeLa/GRIM-19.The protein level of STAT3,p-STAT3,suvivin,ki-67 was reduced too; while the expression of GRIM-19 was increased by protein test.Conclusion The expression of GRIM-19 can slow down the proliferation of cervical cancer cells,suggesting that GRIM-19 may be a new target for the treatment of cervical cancer.

关 键 词：GRIM-19 转染 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]