不同临床分期宫颈癌组织蛋白质组学的比较  

Comparative Proteomic of Cervical Carcinoma Tissues at Differential Clinical Stages

作　　者：朱红[1] 周传意[1] 袁君[1] 曹慧[1] 易琼[1] 刘利[1] 

机构地区：[1]中南大学湘雅医院肿瘤科,长沙410008

出　　处：《肿瘤防治研究》2010年第12期1404-1408,共5页Cancer Research on Prevention and Treatment

基　　金：湖南省自然科学基金资助项目(06JJ4199)

摘　　要：目的通过比较不同临床分期宫颈癌组织的差异表达蛋白,以发现与宫颈癌临床分期相关蛋白,为临床预后和复发的预测提供新指标。方法收集不同临床分期宫颈癌组织,分为早期宫颈癌组和中晚期宫颈癌组,提取组织总蛋白进行二维凝胶电泳,选择部分差异表达蛋白进行MALDI-TOF质谱分析和生物信息学分析,Western blot和免疫组织化学技术检测部分差异表达蛋白HSP70和Galectin-7的表达情况。结果建立了早期宫颈癌组和中晚期宫颈癌组的二维凝胶电泳图谱,其中两组的平均蛋白质点数分别为(1098±23)、(1142±21),通过进行质谱分析和生物信息学查询,鉴定了显著差异表达的12个蛋白点,在早期组上调的4个,包括Hemoglobin subunit beta Hb、caspase-14、Galectin-7、CK19;中晚期组上调的8个,包括Lamin-B1、Flavin reductase、Glutamate de-hydrogenase1、Retinal dehydrogenase1、NMP238、HSP70、Cal modulin-like5、S100A9。Western blot和免疫组织化学检测结果均显示HSP70在中晚期组中的表达高于早期组,Galectin-7在早期组中的表达高于中晚期组。结论不同临床分期的宫颈癌中存在着差异表达蛋白,这些蛋白可能是宫颈癌诊断和预后的生物标志物。Objective Cervical carcinoma clinical stages associated proteins would be found by comparing differential expressed proteins from differential clinical stage cervical carcinoma tissues.MethodsTotal protein from cervical carcinoma tissues was extracted; differential proteome profiles were established and analysized by means of immobilized pH gradient-based two-dimensional polyacrylamide gel electrophoresis（2D-PAGE） and matrix-assisted laser desorption/ionization time of flight mass spectrometry（MALDI-TOF-MS）.HSP70 and Galectin-7 expression were assayed by Western Blot and immunohistochemical technique.Results Well-resolved reproducible 2-DE profiles of human cervical carcinoma tissues were obtained.Average protein dots were（1 098±23）,（1 142±21） at early-stage and medium-advanced stage respectively; By analysis with mass spectrometry and bioinformatics,12 of them were well characterized.4 proteins were over-expressed in early-stage group,including Hemoglobin subunit beta HB,caspase-14,Galectin-7,CK19; 8 protein were over-expressed in medium-advanced stage,including Lamin-B1,Flavin reductase、Glutamate dehydrogenase 1,Retinal dehydrogenase 1,NMP238,HSP70,Calmodulin-like 5,S100A9.HSP70 expressed higher at medium-advanced stage group than at early-stage group and Galectin-7 expressed higher at early-stage group than at medium-advanced group.Conclusion Differential expressed proteins exist in clinical stage of cervical carcinoma,which would be biomarkers for diagnosis and prediction of prognosis.

关 键 词：宫颈癌 蛋白质组学 临床分期 

分 类 号：R737.33[医药卫生—肿瘤] R730.23[医药卫生—临床医学]