铬结合物在铬中毒性肝损害中的作用  

Study on the role of chromiumbinding substances in chromiuminduced hepatic damage

作　　者：曾明[1] 王翔朴[1] 安飞云[1] 高泽宣[1] 

机构地区：[1]湖南医科大学环境医学研究室

出　　处：《中华劳动卫生职业病杂志》1999年第3期164-167,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金

摘　　要：目的了解各种铬结合物与肝损害的关系。方法在铬中毒性肝损害的大鼠模型中，采用ＳｅｐｈａｄｅｘＧ５０凝胶层析技术，对肝中铬结合物进行分离和测定。结果铬染毒大鼠肝组织中，除可检出高分子铬结合物（ＨＭＷＣｒ，相对分子质量（ＭＷ）７００００～１２５００）及低分子铬结合物（ＬＭＷＣｒ，ＭＷ１２５００～５００）外，还发现对照组未检出的小分子铬结合物（ＭＭＷＣｒ，ＭＷ＜５００）。随染毒时间延长，肝损害加重，三者肝中分布变化明显：ＨＭＷＣｒ由２２．２％增至３７．０％；ＭＭＷＣｒ由２．２％增至５．０％；ＬＭＷＣｒ则从７５．６％降至５８．０％。结论ＬＭＷＣｒ可能与铬的解毒有关；ＭＭＷＣｒ的出现并增多反映了机体形成ＬＭＷＣｒ能力的紧张；ＭＭＷＣｒ可能是攻击靶器官的主要活性因素。Objective To study the role of chromiumbinding substances in chromiuminduced hepatic damage. Methods A subacute chromium intoxicated rat model was made and chromiumbinding substances in liver were isolated and measured by sephadex G50 column chromatography and UVAAS. Results Only a high molecular weight chromiumbinding substance (HMWCr,MW700 00012 500) and a low molecular weight chromiumbinding substance (LMWCr,MW12 500500) could be detected in normal animals.But in chromiumexposed animals,a new minimal molecular weight chromiumbinding substance (MMWCr,MW<500) was found in addition to the above constituents.As the exposure time prolonged,hepatic damage aggravated and the distribution of three Crbinding substances was as follows:HMWCr from 22.2% to37.0%,MMWCr from 2.2% to 5.0%,LMWCr from 75.6% to 58.0%. Conclusion LMWCr play a main role in chromium detoxification.The appearance and increase of MMWCr reflected on the insufficiency of LMWCr formation.The MMWCr should be a major active factor on pathogenesis of Crintoxication.

关 键 词：铬中毒性 肝损害 铬结合物 

分 类 号：R575[医药卫生—消化系统] R595[医药卫生—内科学] .2[医药卫生—临床医学]