应用SSR标记进行部分黄肉桃种质鉴定和亲缘关系分析  

Analysis of Genetic Diversity and Relationship of Yellow Flesh Peach by SSR

作　　者：俞明亮[1] 马瑞娟[1] 沈志军[1] 葛志刚[1] 蔡志翔[1] 

机构地区：[1]江苏省农业科学院园艺研究所,南京210014

出　　处：《园艺学报》2010年第12期1909-1918,共10页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30871681);国家科技基础条件平台项目(2005DKA21002-21);农业部种质资源保护项目(NB2010-2130135-7);江苏省科技基础设施建设计划项目(BM2008008)

摘　　要：以116份黄肉桃种质和19份其它桃或桃近缘种种质为试材,利用定位于李属参考图谱上的SSR标记,进行遗传多样性评价及亲缘关系分析。SSR扩增结果表明,筛选出的28对SSR引物共扩增出206个等位基因,其中多态性等位基因159个(占76.56%)。聚类结果表明,在相似系数为0.82时,黄肉桃资源在聚类图上呈两组:美洲、欧洲和亚洲育成黄桃品种聚为一组(Ⅰ),中国地方黄桃品种中唯‘西安杏瓤桃’和‘天津黄肉’出现在这一组;来源于云南、华北、西北地区的中国地方黄桃品种聚为另一组(Ⅱ)。在相似系数为0.87时,育成黄桃品种(Ⅰ)分为8个亚组,美洲育成品种分布在所有8个亚组中,欧洲育成品种分布在Ⅰ-2、Ⅰ-3和Ⅰ-4亚组,而亚洲育成品种仅分布在Ⅰ-3和Ⅰ-4亚组;亲本相同的育成品种在聚类图中临接或出现在较近的位置,育成品种的聚类结果与品种的系谱关系基本吻合。在相似系数为0.87时,中国地方黄桃品种分为7个亚组,西南黄桃和西北黄桃存在一定的组群界限,来自西南的黄桃地方资源主要分布在Ⅱ-1和Ⅱ-5亚组,而来自西北的黄桃地方品种主要出现在Ⅱ-2、Ⅱ-4和Ⅱ-7亚组。品种群的聚类结果表明,亚洲育成品种与美洲育成品种间亲缘关系最近,其次为欧洲育成品种;中国地方黄桃品种与育成品种间亲缘关系较远。In order to evaluate genetic diversity and relationship of yellow flesh peach germplasm, 116 yellow flesh peach varieties,as well as other 19 peach germplasms,were used as plant material. A total of 206 alleles were amplified when screened by 28 SSR primers（26 of them distributed on eight linkage groups on Prunus reference map）. Among all the alleles amplified,159 showed polymorphic with a percentage of polymorphic bands（PPB）of 76.56%. For yellow flesh peaches,two main groups were formed in dendrogram at a similarity coefficient of 0.82,when clustered by NTSYS using UPGMA method. Bred varieties from America,Europe and Asia were clustered into one group（Ⅰ）,and local varieties from China were clustered into another group（Ⅱ）,except‘Xi’an Xingrangtao’and‘Tianjin Huangrou’,which were clustered into bred group. When similarity coefficient was 0.87,bred varieties （Ⅰ）were divided into 8 subgroups. Bred varieties from America were distributed in all the 8 subgroups, and bred varieties from Europe were distributed in three subgroups（Ⅰ-2,Ⅰ-3,andⅠ-4）,while bred varieties from Asia were only distributed in two subgroup（sⅠ-3 andⅠ-4）. Varieties with the same parents were closely clustered,and genetic relationship of bred varieties matched well with pedigree in groupⅠ. When similarity coefficient was 0.87,Chinese local yellow peach varieties were divided into 7 subgroups. Local yellow peaches from southwest China were mainly distributed in subgroup Ⅱ-1 and Ⅱ-5,and those from northwest China were mainly distributed in subgroup Ⅱ-2,Ⅱ-4 and Ⅱ-7. For variety population genetic relationship,bred variety from America was the nearest population to that of Asia, followed by that of Europe. Chinese local yellow peach was far from bred varieties on population dendrogram.

关 键 词：黄桃 遗传多样性 亲缘关系 SSR 

分 类 号：S662.1[农业科学—果树学]