血清9型鸭疫里默氏杆菌在华南地区的分离鉴定  

Isolation and identification of serotype 9 Riemerella anatipestifer in South China

作　　者：冯金牛[1] 陈芳艳[2] 区德庆[3] 阮二垒[1] 杨丽云[1] 黎丁滔[1] 陈瑞爱[3] 唐秀英[3] 王林川[1,3] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]华南农业大学动物科学学院,广东广州510642 [3]广东省大华农动物保健品股份有限公司,广东新兴527400

出　　处：《中国预防兽医学报》2010年第12期937-940,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：广东省科技攻关项目(2006A20301001)

摘　　要：本研究于2009年7月～2010年4月针对广东、广西、福建等养鸭密集地区进行鸭疫里默氏杆菌(RA)流行病学调查,并分离到5个RA分离株。经形态特征、生化特性和血清学鉴定表明,5个分离株与血清9型RA相似。对16S rRNA部分基因进行序列测定,并与GenBank中登录的菌株16S rRNA基因序列进行比对及系统进化分析显示:该5株分离株与报道的RA H-1785、H-2199、H-2565和P-2123的16S rRNA序列相似性较高,同源性达99.1%～99.7%,进一步证实这5个分离株为血清9型RA。Five bacteria were isolated from Guangdong,Guangxi and Fujian during a Riemerella anatipestifer（RA） epidemiological investigation from July 2009 to April 2010 and identified as RA new serotypes（D10,D21,X18,J9 and J33）,These isolates were similar to serotype 9 based on morphological characteristics,biochemical characteristics and serological identification.The 16S rRNA genes were sequenced and compared with those of related strains in GenBank.Phylogenetic analysis showed that isolates had 99.1% to 99.7% similarity to H-1785,H-2199,H-2565 and P-2123 from GenBank.These results confirmed that the isolates of D10,D21,X18,J9 and J33 belonged to serotype 9 RA.It is the first report of serotype 9 RA in South China.

关 键 词：鸭疫里默氏杆菌 血清型 系统进化分析 

分 类 号：S852.61[农业科学—基础兽医学]