IFN-γ联合DC疫苗对口腔鳞癌细胞的抑制作用  

Inhibitory effect of combined IFN-γ and DC vaccine on oral squamous cell carcinoma

作　　者：江琳琳[1] 韩伟[2] 郑勤[1] 王志勇[2] 胡勤刚[2] 张全安[1] 

机构地区：[1]南京市第二医院肿瘤内科,210003 [2]南京市口腔医院口腔颌面外科

出　　处：《江苏医药》2010年第23期2792-2794,I0001,共4页Jiangsu Medical Journal

摘　　要：目的观察干扰素γ(IFN-γ)与树突状细胞(DC)疫苗联合使用后对口腔鳞癌的抑制作用。方法采用RT-PCR方法分析抗原提呈相关基因Tap1和Tapasin在人正常口腔黏膜细胞以及口腔鳞癌细胞系中的表达。采用IFN-γ处理口腔鳞癌细胞系CAL27后,分别在mRNA和蛋白水平检测Tap1和Tapasin的变化。将处理后的肿瘤细胞提取冻融抗原后致敏DC,并将其与同源的T淋巴细胞共培养,诱导出抗原特异性的细胞毒T细胞(CTL),在体外对口腔鳞癌细胞进行杀伤试验。结果与正常人口腔黏膜上皮细胞相比,肿瘤细胞中Tap1和Tapasin的表达明显降低(均在50%以下)。使用IFN-γ处理后,其在mRNA和蛋白水平的表达均明显提高。CTL行体外杀伤实验时发现,IFN-γ处理组肿瘤杀伤效率明显提高,A组(IFN-γ处理48 h)的肿瘤细胞杀伤率达到(87.21±4.67)%,而B组(IFN-γ处理24 h)及C组(IFN-γ处理0 h)则分别达到(73.34±4.52)%和(54.68±4.21)%(P<0.01)。结论 口腔鳞癌细胞中抗原提呈相关基因Tap1和Tapasin的表达明显降低,使用IFN-γ处理后可明显提高其表达。将处理后的肿瘤细胞提取抗原后,诱导出抗原特异性CTL,可显著提高其肿瘤杀伤率。Objective To observe the in vetro inhibitory effect of combined interferon（IFN）-γ and dendritic cell（DC） vaccine on oral squamous cell carcinoma（OSCC）.Methods RT-PCR was used to analyze the expression of antigen-processing machinery（APM） gene Tap1 and Tapasin in both normal oral mucosa epithelium cells and OSCC.CAL27 cell was treated with IFN-γ and the expressions of Tap1 and Tapasin on mRNA and protein levels were detected.The antigen of treated CAL27 was extracted and DC was pulsed.The homologous T lymphocytes were co-cultured with DC to induce antigen-specific cytotoxic T lymphocytes（CTLs）,and in vitro cytotoxic T lymphocyte assay was applied.Results The expressions of Tap1 and Tapasin in OSCC cell lines were decreased significantly（below 50% of normal mucosa epithelium cells）,which were increased significantly after treated by IFN-γ.After adding CTLs,the inhibition rate achieved（87.21±4.67）% in the group IFN-γ-treated for 48 h,which was significantly higher than that in the groups treated for 24 h and 0 h[（73.34±4.52）% and（54.68±4.21）%,respectively]（P0.01）.Conclusion The expressions of Tap1 and Tapasin are decreased in OSCC cells,which can be elevated by IFN-γ.The in vetro inhibitory effect of combined IFN-γ and DC vaccine on OSCC is enhanced when inducing antigen-specific CTLs after extracting tumor antigen from IFN-γ-treated tumor cells.

关 键 词：干扰素Γ 树突状细胞 口腔鳞癌 抗原提呈相关基因 

分 类 号：R739[医药卫生—肿瘤]