大黄鱼肌肉生长抑制素1型和2型基因时空表达分析  

Temporal and Spacial Expression Analysis of MSTN-1 and -2 in Pseudosciaena crocea

作　　者：薛良义[1] 孙升[1] 

机构地区：[1]宁波大学生命科学与生物工程学院,宁波315211

出　　处：《中国细胞生物学学报》2010年第6期885-888,共4页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(No.30871916)资助项目~~

摘　　要：肌肉生长抑制素(MSTN)是动物肌肉生长发育重要的负调控因子。大黄鱼存在两种类型的肌肉生长抑制素基因,利用RT-PCR技术,本文对这两个基因在胚胎发育过程和成体鱼中的组织特异性表达进行了分析。在6月龄和18月龄大黄鱼中,MSTN-1和-2均在多种组织表达,但肌肉组织中只有MSTN-1表达。在胚胎发育过程中,MSTN-1在囊胚期前表达,在原肠胚期未检出,到出膜期又开始表达,而MSTN-2在整个胚胎发育期均未检出。结果提示,无论在成鱼还是在胚胎发育过程中,两种类型的MSTN具有不同的表达调控机制。Myostatin（MSTN） is an important negative regulator in animal muscle growth and development. Large yellow croaker,Pseudosciaena crocea,has two types of MSTN genes,MSTN-1 and -2.Embryonic and tissue-specific expressions of MSTN-1 and -2 genes were analyzed by using RT-PCR.In 6 and 18-month old fish,both MSTN-1 and -2 genes expressed in a wide variety of tissues.However,only MSTN-1 expressed in muscle tissue.In embryonic development,MSTN-1 expressed before blastula stage（6 hpf）,and was undetected throughout gastrulation （9hpf）,and rised again in hatching（27 hpf）.MSTN-2 was undetected through embryogenesis.These results indicated that the expression of MSTN-1 and -2 genes was differentially regulated in both developing embryos and adult tissues of fish.

关 键 词：大黄鱼 肌肉生长抑制素基因 时空表达 

分 类 号：S917.4[农业科学—水产科学]