超高效液相色谱-质谱联用法与气相色谱-质谱联用法分析水性印油印记的主要成分  

Analysis of major components in water based stamp pad inks and their imprints by ultra high performance liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry

作　　者：章晴[1] 邹积鑫[2] 石高军[3] 张丽娟[1] 

机构地区：[1]北京化工大学,北京100029 [2]中国人民公安大学,北京100038 [3]公安部物证鉴定中心,北京100038

出　　处：《色谱》2010年第12期1132-1136,共5页Chinese Journal of Chromatography

基　　金：中央级公益性科研院所基本科研业务费专项资金重点项目(No.2009JB008)

摘　　要：应用超高效液相色谱-质谱联用技术(UHPLC-MS)与气相色谱-质谱联用技术(GC-MS),对环保水性印油中的主要成分(主要颜料与挥发性物质)进行了定性分析。通过超声提取与离心对样品进行预处理后,在ZORBAXEclipse Plus Phenyl-Hexyl(50mm×4.6mm,1.8μm)液相色谱柱,15mmol/L乙酸铵水溶液-乙腈为流动相,在UHPLC-MS负离子电喷雾电离条件下以选择离子监测模式定性分析染料及颜料;采用HP-INNOWAX(30m×0.25mm,0.25μm)气相色谱柱进行GC-MS全扫描,定性分析挥发性物质。研究确认水性印油中的主要颜料成分是酸性红R、水溶曙红Y与颜料红112,主要挥发性物质是甘油、1,2-丙二醇等。本方法快速、准确,可以满足物证鉴定工作中对印记的检测需要,有助于法庭科学中对印油印记的种类区分。Ultra high performance liquid chromatography-mass spectrometry（UHPLC-MS） technology and gas chromatography-mass spectrometry（GC-MS） technology were used to qualitatively analyze the major components in water based stamp pad inks including major colorants and volatile components.After the samples were supersonically extracted and then centrifuged,UHPLC-MS was used to separate and identify the major colorants.A ZORBAX Eclipse Plus Phenyl-Hexyl（50 mm×4.6 mm,1.8 μm） column and 15 mmol/L ammonium acetate-acetonitrile were utilized for the separation and negative selected ion monitoring mode（SIM） was set for the MS analysis.An HP-INNOWAX（30 m×0.25 mm,0.25 μm） column was employed in the GC-MS analysis with the full-scan mode to determine the volatiles.This study demonstrated that the major colorants in the inks and their imprints were Acid Red R,Eosin Y and Pigment Red 112;and the major volatiles were glycerol,1,2-propanediol,etc.The method is rapid and accurate.It also demonstrates that the method can meet the requirements for imprint determination in material evidence identification.The work provides a reliable tool for the categorization research in the forensic sciences.

关 键 词：超高效液相色谱-质谱 气相色谱-质谱 水性印油 印记 

分 类 号：O658[理学—分析化学]