反相高效液相色谱法测定药用植物大红袍中的两个生物活性成分  

Determination of two bio-active compounds in Campylotropis hirtella (Franch.) Schindl.using reversed-phase high performance liquid chromatography

作　　者：谭青[1] 寿清耀[1] 张盛[1] 沈征武[1,2] 

机构地区：[1]上海中医药大学中药学院,上海201203 [2]巴塞利亚药业(中国)有限公司,江苏海门226100

出　　处：《色谱》2010年第12期1150-1153,共4页Chinese Journal of Chromatography

基　　金：国家人事部2007年留学回国人员优秀项目资助

摘　　要：建立了反相高效液相色谱-二极管阵列检测器(RP-HPLC-DAD)测定药用植物大红袍中具有抗菌活性的异黄酮类化合物3′-geranyl-5,7,4′-trihydroxyisoflavone(化合物1)及具有良好免疫抑制活性的紫檀烯类化合物8,9-dihydroxy-1-methoxy-[6′,6′-dimethylpyrano(2′,3′:2,3)]pterocarpene(化合物2)含量的方法。采用的色谱柱为Agilent Zorbax SB-C18柱(250mm×4.6mm,5μm),以乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱,流速为1.0mL/min;柱温30℃。化合物1和化合物2分别在4.4～13.2μg和0.428～1.284μg范围内呈线性关系;平均回收率分别为99.65%和99.11%,相对标准偏差分别为1.83%和2.59%(n=5)。该方法快速简便,灵敏度和分离度好,适用于大红袍药材中活性黄酮类成分的测定。A method of reversed-phase high performance liquid chromatography（RP-HPLC） using diode array detection（DAD） was developed for the quantitative determination of 3′-geranyl-5,7,4′-trihydroxyisoflavone（compound 1） and 8,9-dihydroxy-1-methoxy-[6′,6′-dimethylpyrano（2′,3′： 2,3）]pterocarpene（compound 2） in Campylotropis hirtella.The separation and quantification were achieved using an Agilent Zorbax SB-C18 column（250 mm×4.6 mm,5 μm）,and mobile phases of acetonitrile and 0.1% formic acid with gradient elution at a flow rate of 1.0 mL/min and 30 ℃.The calibration curves for compounds 1 and 2 were linear in the ranges of 4.4-13.2 μg and 0.428-1.284 μg,respectively.The recoveries were 99.65% and 99.11% with the relative standard deviations of 1.83% and 2.59%（n=5）,respectively.This RP-HPLC-DAD method is rather simple,accurate and convenient.It can be used for the quantitative determination of the active flavonoids in Campylotropis hirtella（Franch.） Schindl.

关 键 词：反相高效液相色谱法 异黄酮类化合物 紫檀烯类化合物 大红袍 药用植物 

分 类 号：O658[理学—分析化学]