黄连素对大鼠结肠隐窝细胞钾通道的影响  

Berberine inhibits basolateral potassium current in rat colonic crypt cells

作　　者：尹琬凌[1] 罗和生[2] 韩肇木[3] 

机构地区：[1]武汉市中心医院消化内科,湖北省武汉市430014 [2]武汉大学人民医院消化内科,湖北省武汉市430060 [3]武汉市中心医院内一科,湖北省武汉市430014

出　　处：《世界华人消化杂志》2010年第31期3343-3346,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨黄连素对结肠上皮隐窝细胞基底膜cAMP依赖的钾通道[IK(cAMP)]的影响,以研究其治疗分泌性腹泻的机制.方法:用EDTA溶液分离结肠上皮隐窝细胞,以-60mV为钳制电压钳制细胞,20mV为阶跃,用EPC10膜片钳放大器测量全细胞模式下50、100、500μmol/L的黄连素对结肠上皮细胞基底膜IK(cAMP)的影响.结果:50、100、500μmol/L的黄连素可显著抑制大鼠结肠上皮隐窝细胞基底膜IK(cAMP)(P<0.05),且抑制效应具有浓度依赖性.当阶跃刺激为+80mV时,其IK(cAMP)分别为生理盐水对照组的78.55%±5.72%,60.42%±6.33%,43.78%±6.47%(P<0.05).结论:黄连素能抑制大鼠结肠隐窝细胞基底膜cAMP依赖的钾通道开放,这可能是其治疗分泌性腹泻的机制之一.AIM：To clarify the mechanism behind therapeutic effects of berberine against secretory diarrhea by investigating the effects of berberine on basolateral cAMP-activated potassium current [IK（cAMP）] in rat colonic crypt cells.METHODS：Intact colonic crypt cells were isolated using an EDTA solution.The effects of berberine on IK（cAMP） in isolated colonic crypt cells were detected using the conventional whole-cell patch-clamp technique.RESULTS：Berberine could significantly inhibit IK（cAMP） in rat colonic crypt cells（P〈0.05）.At concentrations of 50,100 and 500 μmol/L,berberine reduced IK（cAMP） to 71.43% ± 3.61%,54.56% ± 5.13% and 38.66% ± 3.85% of that in control cells when depolarizing at ＋80 mV.CONCLUSION：Berberine exerts therapeutic effects against secretory diarrhea possibly by inhibiting IK（cAMP） in rat colonic crypt cells.

关 键 词：黄连素 结肠上皮隐窝细胞 膜片钳 cAMP依赖的钾通道 

分 类 号：R285.5[医药卫生—中药学]