ERK-MAPK信号通路在大鼠颅骨矢状缝体外牵张中的表达  

Activation of ERK-MAPK Signal Pathway in the Stretched Rat Calvarial Sagittal Suture

作　　者：沈庆冉[1] 刘显文[1] 刘人恺[1] 郭婧[1] 邹淑娟[1] 

机构地区：[1]四川大学口腔疾病研究国家重点实验室,四川成都610041

出　　处：《口腔医学研究》2010年第6期777-779,782,共4页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:30772454);四川省科技厅资助课题(编号:2006Z09-013)

摘　　要：目的:了解ERK-MAPK信号通路在颅骨缝牵张成骨中的活化规律。方法:建立大鼠颅骨矢状缝牵张培养模型,运用WesternBlot及免疫组织化学技术,观察p-ERK1/2在骨缝内的表达。结果:在牵张力的作用下,p-ERK1/2表达量于10min达到顶点,随后表达下降并在80min时维持在初始状态的低水平,并且阳性细胞主要集中在骨缝边缘区域。结论:ERK-MAPK信号通路的激活与张应力相关,该通路活化区域和牵张过程中细胞增殖、分化高表达区域一致。表明ERK-MAPK信号通路参与了骨缝内细胞力学信号向生物学信号的转化。Objective：To observe whether the ERK-MAPK signal pathway participates in suture distraction osteogensis. Methods：After the establishment of the organ cultured calvarial suture distraction osteogenesis models,we applied Western blot and immunohistochemisty methods to analysis the expression pattern of ERK-MAPK signal pathway in sutures. Results：Under the distraction,p-ERK1/2 reached the peak in 10min and since then kept in a lower level as the start,and the cells adjacent to the bone edges were mainly p-ERK1/2 positive. Conclusion：The activation of ERK-MAPK signal pathway is related to distraction and may join the process of the conversion from mechanical stress to biology response.

关 键 词：骨缝牵张 ERK-MAPK 信号通路 

分 类 号：R782.2[医药卫生—口腔医学]