Pokemon mRNA与c-myc蛋白在乳腺癌组织中的表达及其相关性  

Expressions of Pokemon mRNA and c-myc in breast carcinoma and their correlation

作　　者：崔明[1] 付朝江[1] 杨彦[1] 冯雁康[1] 刘嘉[1] 

机构地区：[1]成都军区昆明总医院普通外科,昆明650032

出　　处：《中华乳腺病杂志（电子版）》2010年第6期60-63,共4页Chinese Journal of Breast Disease(Electronic Edition)

摘　　要：目的探讨人乳腺癌中原癌基因c-myc和Pokemon mRNA在乳腺癌组织中的表达及其与乳腺癌发生、转移的关系。方法收集本院2008年1月至10月45例浸润性导管癌组织标本、20例癌旁乳腺组织标本和20例正常乳腺组织标本,均为女性,年龄23～69岁。所有标本均经临床病理确诊。采用原位杂交法检测Pokemon mRNA表达,采用免疫组织化学法检测c-myc表达。采用卡方检验和等级相关法进行统计分析。结果 (1)Pokemon mRNA的阳性表达率在乳腺癌组织、癌旁乳腺组织和正常乳腺组织中分别是71.11%(32/45),30.00%(6/20)和20.00%(4/20),3者间差异有统计学意义(P=0.000)。c-myc阳性表达率在乳腺癌组织、癌旁乳腺组织和正常乳腺组织中分别是86.67%(39/45),60.00%(12/20)和50.00%(10/20),3者间差异有统计学意义(P=0.004)。(2)乳腺癌细胞质Pokemon mRNA表达和乳腺癌细胞质c-myc表达都与乳腺癌腋窝淋巴结转移有关(P=0.012,P=0.027)。(3)乳腺癌细胞质Pokemon mRNA表达与c-myc表达有关联性(r=0.585,P<0.000)。结论 Pokemon mRNA和c-myc蛋白可能参与乳腺导管癌的发生和转移。Pokemon mRNA表达和c-myc表达两者间有相关性。Objective To explore the expressions of Pokemon mRNA and proto-oncogene c myc in breast cancer and their relation with carcinogenesis and metastasis. Methods From January to October 2008, 45 samples of invasive ductal carcinoma, 20 samples of pericancer tissue and 20 samples of normal breast tissue were collected for the study. All study subjects were female, aged 23 to 69 years. All samples were confirmed pathologically. The expression of Pokemon mRNA was examined using in situ hybridization and the expression of c myc was tested using immunohistochemistry. Chi square test and Spearmaffs rank correlation coefficient were used for statistical analysis. Results （1）The rate of positive expression of plasma Pokemon mRNA in breast cancer tissue, pericancer tissue and normal breast tissue was 71.11% （32/45）, 30. 00% （6/20） and 20. 00% （4/20） respectively, with statistical differenee between the three groups （P=0. 000）. The rate of positive expression of c-myc in breast cancer tissue, pericancer tissue and normal breast tissue was 86.67% （39/45）, 60. 00% （12/20） and 50.00% （10/20） respectively, with statistical difference between the three groups （P = 0. 004 ）. （2）The positive expressions of Pokemon mRNA and c myc in breast cancer were correlated with breast cancer axillary lymph node metastasis （P=0.012,P=0.027）. （3）There was a positive correlation between Pokemon mRNA and c-myc （r=0. 585, P〈0. 000）. Conclusions Pokemon mRNA and c myc participate in the pathogenesis and metastasis of breast cancer, and there is a correlation between Pokemon mRNA and c-myc.

关 键 词：乳腺癌 POKEMON MRNA C-MYC蛋白 原位杂交 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]