竹笋醇提取物对酪氨酸酶活力的影响  

Effects of Ethanol Extracts from Bamboo Shoot on Activity of Tyrosinase

作　　者：王文文[1] 袁艺[1] 武静文[1] 

机构地区：[1]安徽农业大学生命科学学院,合肥安徽230036

出　　处：《江苏农业学报》2010年第6期1197-1200,共4页Jiangsu Journal of Agricultural Sciences

基　　金："十一五"国家科技支撑项目(2007DAQ01049;2008BADA9B04)

摘　　要：用L-酪氨酸和邻苯二酚作底物,以曲酸、熊果苷为对照,测定不同种、不同季节竹笋笋肉、笋壳醇提取物对酪氨酸酶单酚酶和双酚酶活力的抑制作用。结果表明:麻竹笋肉醇提物浓度为1.0 mg/ml时对酪氨酸酶单酚酶的抑制作用最大,抑制率为45.97%,与0.1 mg/ml曲酸相接近,高于0.1 mg/ml熊果苷;醇提物对酪氨酸酶单酚酶的抑制作用从强到弱依次是:麻竹笋肉>毛竹春笋肉>麻竹笋壳>毛竹春笋壳>毛竹冬笋壳>毛竹冬笋肉>雷竹笋壳>雷竹笋肉。麻竹笋壳醇提物浓度为1.0 mg/ml时对双酚酶的抑制作用最大,抑制率为30.05%,高于0.08mg/ml熊果苷和0.08 mg/ml曲酸;醇提物对双酚酶的抑制作用从强到弱依次是:麻竹笋壳>麻竹笋肉>毛竹春笋壳>毛竹冬笋壳>毛竹冬笋肉>雷竹笋壳>毛竹春笋肉>雷竹笋肉。The inhibitions of ethanol extracts from bamboo shoots on monophenolase and diphenolase of tyrosinase were tested,taking kojic acid and arbutin as control,and L-Tryosine and pyrocatechin as substrates.The results showed that at the concentration of 1.0 mg/ml,ethanol extract of Latiflorus mcclure flesh displayed the strongest inhibition against tyrosinase monophenolase,with inhibitory rate of 45.97%,close to that in 0.1 mg/ml kojic acid treatment,and higher than that in 0.1 mg/ml arbutin treatment.The order of inhibitory effects of ethanol extracts on tyrosinase monophenolase was： Latiflorus mcclure flesh Phyllostachys pubescens flesh in spring Latiflorus mcclure shell Phyllostachys pubescens shell in spring Phyllostachys pubescens shell in winter Phyllostachys pubescens flesh in winter Phyllostachys pracecox shell Phyllostachys pracecox flesh.At the concentration of 1.0 mg/ml,ethanol extract of Latiflorus mcclure shell showed the highest inhibition action on tyrosinase diphenolase,with inhibitory rate being 30.05%,higher than those in 0.08 mg/ml kojic acid and 0.08 mg/ml arbutin treatments.The order of inhibitory effects of ethanol extracts on tyrosinase diphenolase was： Latiflorus mcclure shell Latiflorus mcclure flesh Phyllostachys pubescens shell in spring Phyllostachys pubescens shell in winter Phyllostachys pubescens flesh in winter Phyllostachys pracecox shell Phyllostachys pubescens flesh in spring Phyllostachys pracecox flesh.

关 键 词：酪氨酸酶 竹笋 抑制作用 

分 类 号：S644.2[农业科学—蔬菜学]