液芯胶囊制备因素对保加利亚乳杆菌释放性能的影响  

Effect of Preparation Conditions of Liquid-core Encapsulation on Releasing Property of Lactobacillus bulgaricus

作　　者：王英[1] 周剑忠[1] 卢俭 黄开红[1] 张志辰[1] 刘小莉[1] 

机构地区：[1]江苏省农业科学院农产品加工研究所,江苏南京210014 [2]南京奶业集团有限公司,江苏南京210095

出　　处：《江苏农业学报》2010年第6期1378-1381,共4页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省科技支撑计划项目(BE2008368);国家"863"计划项目(2006AA10Z343)

摘　　要：分析壳聚糖乙酸溶液浓度、海藻酸钠溶液浓度、氯化钙溶液浓度和成膜时间4种因素对液芯微胶囊中保加利亚乳杆菌的释放性能的影响。试验以改变1种影响因素,固定其他影响因素的方法制备包裹保加利亚乳杆菌的液芯微胶囊,将其置MRS培养基上于37℃培养箱中培养,定时取样,用分光光度法分析培养液中保加利亚乳杆菌的菌体含量。结果显示:壳聚糖乙酸溶液浓度、氯化钙溶液浓度和成膜时间对保加利亚乳杆菌的释放性能均有很大影响;当海藻酸钠溶液浓度满足钙离子的配位需求后,其浓度对保加利亚乳杆菌的释放性能影响不大。表明通过改变液芯胶囊组分的浓度和成膜时间可以控制液芯胶囊的通透性,从而改变保加利亚乳杆菌的释放性能,对实现控制培养液中菌体密度具有重要意义。To study the effects of four kinds of factors（chitosan concentration,sodium alginate concentration,CaCl2 concentration and formed formation time） on the releasing property of Lactobacillus bulgaricus,liquid-core encapsulation with Lactobacillus bulgaricus made by changing one factor and fixing others,were cultured on MRS medium at 37 ℃ in incubator.The contents of Lactobacillus bulgaricus in liquid-core encapsulation taken at regular intervals was determined by spectrophotometry.The chitosan concentration,CaCl2 concentration and film formation time had great effects on the permeability of liquid-core encapsulation.When the concentration of sodium alginate was fit to calcium coordination requirement,it had little effect on the releasing property of Lactobacillus bulgaricus.As a whole,the change of the component concentration and film formation time of liquid-core encapsulation can alter its permeability,thus altering the releasing property of Lactobacillus bulgaricus,which is beneficial for control the density of Lactobacillus bulgaricus in media.

关 键 词：液芯胶囊 保加利亚乳杆菌 释放性能 通透性 

分 类 号：TS201.3[轻工技术与工程—食品科学]