姜黄素对荷瘤裸鼠皮下移植瘤的影响  

Effect of curcumin on subcutaneous transplantation tumor of human hepatoma in nude mice

作　　者：江敏华[1] 谢莹[1] 胡凤霞[1] 甘建和[1] 

机构地区：[1]苏州大学附属第一医院感染病科,江苏苏州215006

出　　处：《实用临床医药杂志》2010年第12期7-11,共5页Journal of Clinical Medicine in Practice

摘　　要：目的观察姜黄素在体内对人肝癌细胞株SMMC-7721的抗肿瘤作用。方法用姜黄素在SMMC-7721肝癌细胞荷瘤裸鼠的瘤体内进行注射治疗,12 d后处死裸鼠,摘除瘤体,称瘤重,观察肿瘤生长变化,并通过免疫组化法检测Bcl-2、Bax、Caspase-3等与细胞凋亡相关因子的表达。结果姜黄素可抑制SMMC-7721细胞对裸鼠的致瘤能力,肿瘤体积较对照组显著减小(P<0.01),质量也明显小于对照组(P<0.01),肿瘤生长抑制率达47.7%,免疫组化结果显示阿的平能明显上调与细胞凋亡相关因子Bax和Caspase-3的表达和下调Bcl-2和Survivin的表达。结论姜黄素能抑制SMMC-7721细胞的体内致瘤能力。Objective To study the anti-tumor effects of curcumin on human hepatocellular carcinoma SMMC-7721 Cells in vivo. Methods For in vivo study, we first established SMMC- 7721 tumor model by grafting SMMC-7721 cells in athymic nude mice, and then injected curcumin into the tumors. 12 days after injection, we sacrificed the mice, removed the tumors, weighed and calculated the ratios of tumor-suppression. We also detected the expressions of Bcl-2, Bax, Caspase -3 and Survivin with immumohistochemistry. Results The tumorigenicity of SMMC-7721 cells was significantly inhibited by curcumin. The size of the tumor in the curcumin treatment group was significantly smaller than that in the control group （P K 0.01）, and the tumor weight was also markedly reduced in curcumin group than that in the control group [ （0. 485 ± 0. 178） g vs （0. 928 ± 0. 223） g, （P〈0.01） ]. The tumor growth inhibition rate was 47.7%. The in vivo data showed that curcumin suppressed the tumor growth conspicuously through down-regulating the expressions of bcl-2 and Survivin and up-regulating the expressions of bax and easpase-3. Conclusion Curcumin inhibits the in vivo tumorigenicity of SMMC-7721 cells.

关 键 词：姜黄素 凋亡 SMMC-7721细胞 体内成瘤 

分 类 号：R735.7[医药卫生—肿瘤]