2型糖尿病伴慢性牙周炎患者牙周可疑致病菌的定植研究  

Study of putative periodontal pathogens colonies in type 2 diabetes with chronic periodontitis

作　　者：马丽[1] 潘亚萍[1,2] 张建全[2] 

机构地区：[1]中国医科大学口腔医学院口腔生物学教研室,辽宁沈阳110002 [2]中国医科大学口腔医学院牙周科,辽宁沈阳110002

出　　处：《上海口腔医学》2010年第6期611-615,共5页Shanghai Journal of Stomatology

基　　金："十一五"国家科技支撑计划课题(2007BAI18B02);辽宁省科学技术计划项目(2007225007)~~

摘　　要：目的:研究2型糖尿病伴慢性牙周炎患者龈下菌群的定植,进一步探讨牙周可疑致病菌在慢性牙周炎与2型糖尿病关系中的作用。方法:采集90例重度慢性牙周炎及30例牙周健康的2型糖尿病患者的龈下集合菌斑,以牙周可疑致病菌牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)、放线共生放线杆菌(Aggregatibacer actinomycete mcomitans,Aa)、具核梭杆菌(Fusobacterium nucleatum,Fn)、中间普氏菌(Prevotella intermedia,Pi)、福塞坦氏菌(Tannerella forsythia,Tf)、牙垢密螺旋体(Treponema denticola,Td)为目标菌,应用以16SrRNA为基础的聚合酶链反应(PCR)技术对龈下菌群进行检测,并测定PCR产物电泳条带的灰度值,以计算各种细菌在菌斑中所占的比例。采用t检验、χ2检验、Pearson相关等统计方法,分析患者空腹(FBG)及糖耐量实验2h血糖(OGTT2hBG)水平,血浆总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C)水平与细菌种类和细菌在菌斑中所占比例的关系。结果:Tf阳性患者的TC水平显著高于阴性患者,Tf在菌斑中所占比例与空腹血糖水平显著正相关,P=0.002;Pg在菌斑中所占比例与TC显著正相关,P=0.035;Pi在菌斑中所占比例与空腹血糖和血浆总胆固醇(TC)水平显著负相关,P分别为0.003、0.041。结论:2型糖尿病对某些牙周可疑致病菌的定植有一定影响。PURPOSE：To study the permanent planting of periodontal pathogens in the subgingival plaque of periodontitis patients with type 2 diabetes,and evaluate role of periodontal pathogens in chronic periodontitis and type 2 diabetes.METHODS：One hundred and twenty subgingival plaque samples were collected from 90 severe periodontitis and 30 healthy periodontium patients with type 2 diabetes.Porphyromonas gingivalis（Pg）,Aggregatibacer actinomycetemcomitans（Aa）,Fusobacterium nucleatum（Fn）,Prevotella intermedia（Pi）,Tannerella forsythia（Tf） and Treponema denticola（Td） were taken as aim pathogens,polymerase chain reaction（PCR） detection was used to determine the prevalence of Pg,Aa,Fn,Pi,Tf,Td in the subgingival plaque samples.The average gradation（AVG）of positive band in the agarose gel electrophoresis was analyzed to determine the proportion of Pg,Aa,Fn,Pi,Tf,Td in the subgingival plaque.Chi-square test,t test and Pearson correlation were used to analyze the relationship between the periodontal pathogens and blood glucose and blood lipid.RESULTS：The level of triglyceridey（TC） in Tf positive subjects was significantly higher than that in Tf negative subjects,the proportion of Tf in subgingival plaque was positively correlated with the value of fasting blood glucose（FBG）.The proportion of Pg was positively correlated with the level of TC.The proportion of Pi was negatively correlated with the value of FBG and TC.CONCLUSION：Type 2 diabetes can affect the permanent planting of some putative periodontal pathogens.

关 键 词：2型糖尿病 牙周可疑致病菌 血糖 血脂 

分 类 号：R781.4[医药卫生—口腔医学]