维生素A对牙周致病菌免疫小鼠免疫应答调节的作用  

Effect of vitamine A on mice immune response induced by specific periodontal pathogenic bacteria-immunization

作　　者：林晓萍[1,2,3] 周晓佳[1,2] 刘洪利[1,2] 杜莉莉[1,2] Toshihisa Kawai 

机构地区：[1]中国医科大学附属盛京医院口腔科,辽宁沈阳110004 [2]辽宁省口腔医学研究所牙周病研究室,辽宁沈阳110001 [3]Department of Immunology,The Forsyth Institute,Boston,MA,02115,USA

出　　处：《上海口腔医学》2010年第6期630-634,共5页Shanghai Journal of Stomatology

基　　金：辽宁省自然科学基金(20092139);沈阳市科学技术项目计划(F10-149-9-32)~~

摘　　要：目的:探讨维生素A缺乏对特异性牙周致病菌—伴放线放线杆菌(A.actinomycetetemcomitans,Aa)引起的小鼠免疫应答作用的影响。方法:整个实验过程采用无维生素A饮食(vitamineA-depleted diet,VAD)或常规维生素A饮食(vitamine A-sufficient regulardiet,RD)喂养BALB/c鼠。2周后,免疫Aa建立免疫动物模型,6周后处死小鼠,ELISA法测定血清中的抗Aa特异性抗体总IgG、IgM及IgG亚类抗体滴度及细胞上清中细胞因子的浓度,3H-Tdr掺入法测定T细胞增殖反应。实验结果采用SPSS11.5软件包进行统计学分析。结果:Aa免疫的总IgG和IgM抗体水平明显升高,非免疫组则不能产生抗体。Aa免疫+VAD组与Aa免疫+RD组相比,总IgG水平显著升高(P<0.05);IgG2a的抗体水平明显增加,而IgG1亚型的抗体水平却明显降低,差异显著(P<0.05)。Aa免疫组可诱导机体产生较强的特异性T细胞免疫反应,而Aa免疫+VAD组T细胞增殖反应明显高于Aa免疫+RD组,具有统计学差异(P<0.05);细胞上清中RANKL、IFN-γ及TNF-α的表达增加,IL-10的表达降低(P<0.05)。结论:饮食中维生素A缺乏,可增加Aa免疫鼠引起的免疫炎症反应,提示充足的维生素A是维持机体健康的重要因素。PURPOSE：The aim of this study was to investigate the effect of vitamine-A deficiency on the induction of specific periodontal pathogenic bacteria A.actinomycetetemcomitans（Aa） immunization.METHODS：BALB/c mice were fed with vitamine A-depleted diet or control regular diet throughout the whole experiment period.After 2 weeks,immunized formalin-killed Aa to build immunized models,6 weeks later,sacrificed to determine specific antibody—IgG,IgM and sub-class IgG antibody titers in serum,and concentration of IL-10,IFN-γ,TNF-α and RANKL in T cell supernatant were measured by ELISA and T cell proliferation was measured by cintilography.SPSS 11.5 software package was used for statistical analysis.RESULTS：The levels of whole IgG and IgM antibody which were immunized by Aa significantly elevated,non-immune group was unable to produce any antibody.Compared with Aa immunized＋RD group,the level of whole IgG in Aa immunized＋VAD group was significantly higher（P0.05）;The levels of IgG2a increased obviously,whereas the levels of IgG1 subtype antibody conspicuous decreased,with a significant difference（P0.05）.Aa immunized group could induce body to produce a strong specific T-cell immune response,but Aa immunized＋VAD group had a higher T cell proliferate response compared with Aa immunized＋RD group,with a statistically significant difference（P0.05）;The expression of RANKL,IFN-γ and TNF-α supernatant increased,while the expression of IL-10 decreased（P0.05）.CONCLUSIONS：The lack of vitamin-A diet can increase the immunized mice＇s susceptibility to periodontal pathogenic bacteria and trigger or aggravate immune inflammatory response.Adequate vitamin A is an important factor in maintaining body health.

关 键 词：维生素A 细胞因子 T淋巴细胞 伴放线放线杆菌 免疫应答 

分 类 号：R781.4.2[医药卫生—口腔医学]