不同浓度的含氟牙膏再矿化根面龋的体外实验  

Remineralization of artificial root caries by fluoride dentifrice of different fluoride concentration in vitro

作　　者：刘红春[1] 俞少杰[2] 胡德渝[3] 

机构地区：[1]中山大学光华口腔医学院口腔预防科,广东广州510055 [2]中山大学光华口腔医学院牙周科,广东广州510055 [3]四川大学华西口腔医学院口腔预防科,四川成都610041

出　　处：《上海口腔医学》2010年第6期653-657,共5页Shanghai Journal of Stomatology

摘　　要：目的:比较氟浓度分别为1.1g/L、1.45g/L和5g/L的含氟牙膏促进根面龋再矿化的效果。方法:取第三磨牙靠近冠方的根部制备成牙根组织块,开窗后,置37℃脱矿液中96h,随机分为1.1g/L、1.45g/L、5g/L含氟牙膏组和不含氟牙膏组,每组8个样本,置于处理液、酸性缓冲液和中性缓冲液中,进行再矿化循环。每天6次,循环8天。应用电阻抗仪测量开窗区再矿化前后的电阻值,偏光显微镜观察各组样本再矿化后的形态学变化,激光扫描共聚焦显微镜(CLSM)分析样本再矿化后矿物质含量的变化。采用SPSS13.0软件包对数据进行单因素方差分析。结果:再矿化后含氟牙膏组样本的电阻值变化、CLSM总荧光量和平均荧光量变化均高于不含氟牙膏组,且5g/L含氟牙膏组的变化高于1.1g/L和1.45g/L组(P<0.05)。偏光显微镜观察发现,含氟牙膏组样本再矿化后可见不同程度的矿物质沉积,5g/L含氟牙膏组的矿物质沉积带宽于1.1g/L和1.45g/L组。结论:含氟牙膏能有效再矿化人工根面龋,氟浓度为5g/L的牙膏再矿化效果优于氟浓度为1.1g/L和1.45g/L的牙膏。PURPOSE：Although the effectiveness of fluoride dentifrice in remineralizing enamel caries has been established,the optimum fluoride concentration for remineralization root caries has not been defined.The aim of this study was to assess and compare the effect of 1.1g/L,1.45g/L and 5g/L fluoride dentifrices in remineralization of artificial root caries.METHODS：Sound human tooth fragments obtained from the cervical portion of root were stored in a demineralization solution for 96h at 37℃ to induce artificial root caries lesions.The fragments were then randomly divided into four treatment groups including 1.1g/L,1.45g/L,5g/L fluoride dentifrice group and non-fluoride dentifrice control group.The demineralized samples were pH-cycled through treatment solutions,acidic buffer and neutral buffer for 8 days at 6 cycles per day.The samples were evaluated using electrical caries monitor（ECM）,polarized light microscopy（PLM） and confocal laser scanning microscopy（CLSM）.The data was analyzed using ANOVA with SPSS13.0 software package.RESULTS：Variations of LgECM,total fluorescence and average fluorescence from CLSM after remineralization in fluoride dentifrice groups were significantly higher than those in the control group（P0.05）;Variations of LgECM,total fluorescence and average fluorescence from CLSM after remineralization in the 5g/L fluoride dentifrice group were significantly higher than those in the 1.1g/L and 1.45g/L fluoride dentifrice groups（P0.05）.PLM observation showed that a banding or deposit of mineral appeared within the lesions of specimens in the fluoride dentifrice groups.A wider mineral band was seen in the 5g/L fluoride dentifrice group than in the 1.1g/L and 1.45g/L fluoride dentifrice groups.CONCLUSIONS：Fluoride dentifrice is effective in remineralizing artificial root caries and 5g/L fluoride dentifrice has better effect than 1.1g/L and 1.45g/L fluoride dentifrices.

关 键 词：牙根龋 电阻抗 牙再矿化 含氟牙膏 

分 类 号：R781.1[医药卫生—口腔医学]