薄层色谱-气相色谱法测定人肝癌细胞BEL-7404中芥酸甲酯的含量及意义  

Determination of concentration of erucic acid methyl ester in human hepatocellular line BEL-7404 by TLC-GC and its significance

作　　者：秦箐[1,2] 林华[2] 王闻楚[2] 高明星[2] 李海冬[2] 邝晓聪[2] 

机构地区：[1]广西大学化学化工学院,南宁530021 [2]广西医科大学

出　　处：《山东医药》2010年第47期7-8,共2页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30560186);广西壮族自治区科学技术厅科学基金资助项目(0728128;0991147)

摘　　要：目的探讨测定人肝癌细胞BEL-7404内芥酸甲酯浓度的方法。方法采用薄层色谱一气相色谱法测定空白细胞裂解液、加药细胞裂解液、加药细胞培养液内芥酸钾酯浓度，并确定芥酸甲酯浓度和峰面积之间的关系。结果芥酸甲酯50-5000μg/ml具有良好的峰面积-浓度线性关系（r=0．99995）；芥酸甲酯微乳液进人到人肝癌细胞BEL-7404内的量为24．9％。结论薄层色谱-气相色谱法能够准确测定药物进人细胞的浓度。其操作简单快速、干扰小。Objective To investigate the method for the determination of concentration of erucic acid methyl ester in human hepatocellular line BEL-7404. Methods The concentration of erucie acid methyl ester in the blank cell lysate, the medium and the lysate of cells that added erucic acid methyl ester were detected by thin layer chromatography-gas chromatography（TLC-GC） ,and the relationship between the concentration of erotic acid methyl ester and the peak area. Results A good linear correlation between peak area and concentration were obtained within a concentration range of 50 ~ 5 000 μg/ml（ r = 0. 999 95）. The amount of erucic acid methyl ester microemulsion got into human hepatocellular line BEL-7404 were 24.9%. Conclusions TLC-GC can determine the concentration of the drug in the cell accurately;this method is simple, rapid and stability.

关 键 词：薄层色谱-气相色谱法 肝癌BEL-7404细胞 芥酸甲酯 

分 类 号：R735.7[医药卫生—肿瘤]