土槿乙酸对不同人肿瘤细胞系的抑制作用及机制  

Antitumor effect and mechanism of pseudolarix acid B on various human tumor cells

作　　者：孟爱国[1] 刘春艳[2] 

机构地区：[1]华北煤炭医学院附属医院,河北唐山063000 [2]华北煤炭医学院药学系

出　　处：《山东医药》2010年第47期20-22,共3页Shandong Medical Journal

基　　金：河北省科技厅资助项目(07275521);唐山市科技局资助项目(08130204A-115)

摘　　要：目的探讨土槿乙酸(PLAB)对人不同肿瘤细胞系的抑瘤作用及其机制。方法将MGC803、AGS、SMMC7721、LOVO、A375、SK-28和624 mel细胞培养后,取对数生长期细胞,采用不同浓度PLAB干预;采用MTT法检测肿瘤细胞存活率;RT-PCR法检测细胞内过氧化物酶体增殖物活化受体γ(PPARγ)mRNA表达;流式细胞术检测细胞周期变化。结果 PLAB干预后肿瘤细胞存活率明显降低,呈时间和剂量依赖性(P均<0.05);PPARγmR-NA在MGC803细胞中表达最强,SMMC7721细胞中次之,LOVO细胞中表达最低;PLAB(10μmol/L)作用肿瘤细胞后,AGS、MGC803、SK-28细胞G2/M期细胞百分比明显增加(P<0.05)。结论 PLAB在体外能明显诱导肿瘤细胞G2/M期阻滞,在表达野生型p53的肿瘤细胞,可能与抑制p53表达有关;在表达突变型p53的肿瘤细胞,可能与激活PPARγ表达有关。Objective To evaluate the antitumor effect of pseudolarix acid B （PLAB） on various human tumor cell lines and its mechanism in vitro. Methods MGC803,AGS,SMMC7721 ,LOVO, A375,SK-28 and 624 reel in logarithmic growth phase were intervented with different concentrations of PLAB. Tile survival rate of tumor cells were tested by MTT; The expression of peroxisome proliferator activated receptorγ（PPARγ） mRNA was detected by RT-PCR; Cell cycle was an- alyzed by flow eytometry. Results The survival rate of tumor ceils decreased significantly after the intervene of PLAB in a dose and time-dependent manner（ all P 〈 0.05 ）. The expression of PPARγ mRNA was the strongest in MGC803 cells, the secondary in SMMC7721 cells, the lowest in LOVO cells. After the treatment of PLAB （ 10 μmol/L） ,the cell percentage of AGS,MGC803 and SK-28 in the G2/M phase inereased obviously. Conclusions PLAB can significantly induce G2 / M phase arrest on tumor cells in vitro, which may be related to the inlfihition of p53 expression in the tumor cells expressing wild-type p53, and be related to the activation of PPARγ expression in the tumor cells expressing mutant p53.

关 键 词：土槿乙酸 P53 过氧化物酶体增殖物活化受体Γ 细胞周期 

分 类 号：R730.231[医药卫生—肿瘤]