原发性肝癌组织中DLK1表达及意义  

Expression and significance of DLK1 in primary hepatocellular carcinoma tissues

作　　者：张艳丽[1] 康凯夫[1] 

机构地区：[1]南方医科大学附属顺德第一人民医院,广东顺德528300

出　　处：《山东医药》2010年第47期26-28,共3页Shandong Medical Journal

基　　金：广东省社会发展领域科技计划项目(200883113)

摘　　要：目的探讨DLK1在原发性肝细胞性肝癌(HCC)发生、发展中的作用。方法分别应用免疫组化SP法和原位杂交的方法检测150例原发性肝细胞癌、50例肝硬化、50例肝炎组织中和10例意外死亡(生前体健)者肝组织中DLK1表达。结果 DLK1蛋白及DLK1 mRNA在HCC中的表达明显高于肝硬化?肝炎和正常肝组织表达(P均<0.05)。DLK1蛋白表达与肿瘤直径及临床分期有明显相关性(P均<0.05)。结论 DLK1高表达可促进HCC的发生、发展。Objective To investigate the roles of DLK1 in the occurrence and development of primary hepatocellular earcinoma（HCC）. Methods The expression of DLK1 were detected by immunohistoehemical method and in situ hybrid- ization in 150 HCC tissues, 50 cirrhosis tissues, 50 hepatitis tissues and 10 normal tissues. Results The expression level of DLK1 protein and DLK1 mRNA in HCC tissues were significantly higher than those in the cirrhosis tissues, hepatitis tis- sues and normal tissues （ all P 〈 0.05 ）. The expression of DLK1 protein were signifieantly correlated to the tumor diameter and clinical stages （both P 〈 0.05）. Conclusion Overcxpression of DLK1 can improve the occurrence and development of HCC,

关 键 词：DLK1基因 原发性肝细胞肝癌 免疫组织化学 原位杂交 

分 类 号：Q78[生物学—分子生物学] R735.7[医药卫生—肿瘤]