单纯疱疹病毒2型感染的细胞培养系统的建立  

Establishing of the HSV-2 infected cell culture system

作　　者：梁义[1] 林有坤[2] 梁铭[2] 

机构地区：[1]柳州市柳铁中心医院皮肤科,广西壮族自治区柳州540047 [2]广西医科大学第一附属医院皮肤科

出　　处：《中国基层医药》2010年第1期11-13,I0004,共4页Chinese Journal of Primary Medicine and Pharmacy

基　　金：广西自然科学基金资助项目（桂科0135036）

摘　　要：目的建立稳定的单纯疱疹病毒2型（HSV-2）感染的细胞培养系统，为研究药物的抗HSV-2作用搭建一个良好的平台。方法以猴肾细胞（Vero）为繁殖细胞，选择人鼻咽癌上皮细胞（Hep-2）为靶细胞，观察HSV-2在Hep-2株中的致细胞病变效应（CPE）。结果HSV-2感染Vero细胞4d后大量繁殖；HSV-2感染Hep-2细胞12h后可出现明显的细胞病变。结论以Vero为繁殖细胞，Hep-2为靶细胞的细胞培养系统是研究药物抗HSV-2作用的良好的平台。Objective To establish a HSV-2 infected cell culture system, so as to confirm the suppressing effect of medicine on HSV-2 including effective concentration and safety. Methods A monkey kidney cell line, Vero ,and a human tracheal epithelial cell line,Hep-2 were used in HSV infected cell culture system. HSV-2 was propagated in Veto cells and Hep-2 cells was served as targets of HSV-2 infection. Then eytopathic effect （ CPE ） was performed. Results HSV-2 was propagated abundance in Vero cells after 4 days;The CPE occurred in Hep-2 cells after 12 hours infected by HSV-2. Conclusion The HSV-2 infected cell culture system we established was stable for utilization. As well as to confirm the suppressing effect of medicine on HSV-2.

关 键 词：单纯疱疹病毒2型 细胞培养技术 

分 类 号：R73[医药卫生—肿瘤]