微弧氧化-碱热处理后钛铌锆锡合金种植体-骨界面的扫描电镜观察  

Scanning electron microscopy observation of the growth of osteoblasts on Ti-24Nb-4Zr-8Sn modified by micro-arc oxidation and alkali-heat treatment and implant-bone interface

作　　者：韩雪[1] 刘洪臣[1] 王东胜[1] 李述军[2] 杨锐[2] 

机构地区：[1]中国人民解放军总医院口腔医学研究所,北京100853 [2]中国科学院金属研究所

出　　处：《中华口腔医学杂志》2011年第1期50-53,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金（30973354）

摘　　要：目的 通过观察微弧氧化-碱热处理后钛铌锆锡合金（Ti-24Nb-4Zr-8Sn）表面成骨细胞的生长情况和种植体-骨界面的结合情况,探讨对钛铌锆锡合金表面应用微弧氧化-碱热处理进行改性的效果.方法 用钛铌锆锡合金制备圆片形试件和圆柱形种植体各12个,均分为3个组（空白对照组、微弧氧化组和微弧氧化-碱热处理组）进行相应表面处理.将MC3T3-E1成骨样细胞接种于圆片形试件表面,培养3 d后扫描电镜（SEM）比较各组试件表面的细胞形态.将圆柱形种植体植入犬胫骨内,3个月后SEM观察种植体-骨界面的微观形貌.结果 微弧氧化和微弧氧化-碱热处理均可使钛铌锆锡合金表面生成具有生成微孔结构的氧化膜,后者试件表面的细胞与微孔嵌合更紧密,种植体与骨组织形成良好的骨结合.结论 碱热处理可进一步完善微弧氧化方法,提高种植体与骨界面的结合.Objective To observe the efficacy of micro-arc oxidation and alkali-heat treatment （MAH） on Ti-24Nb-4Zr-8Sn（Ti2448）. Methods Disks（ diameter of 14. 5 mm, thickness of 1 mm） and cylinders （diameter of 3 mm, height of 10 mm） were fabricated from Ti2448 alloy. Samples were divided into three groups： polished（ Ti2448 ）, micro-arc oxidation（ MAO-Ti2448 ）, micro-arc oxidation and alkali-heat treatment（MAH-Ti2448）. MC3T3-E1 osteoblastic cells were cultured on the disks and cell morphology was observed with scanning electron microscopy（SEM） aftre 3 days. The cylinder samples were implanted in the tibia of dogs and implant-bone interface was observed with SEM after 3 months. Results A rough and porous structure was shown in both MAO and MAH group. The MC3T3-E1 cells on the MAH-Ti2448 discs spread fully in intimate contact with the underlying coarse surface through active cytoskeletal extentions.Osseointegration was formed in the implant-bone interface in MAH samples. Conclusions MAH treatment can provide a more advantageous Ti2448 surface to osteoblastic cells than MAO treatment does, and the former can improve the implant-bone integration.

关 键 词：钛 显微镜检查 电子 扫描 微弧氧化 碱热处理 

分 类 号：R783[医药卫生—口腔医学]