氯磺酚N蛋-白质-TX-100体系的吸收光谱及其分析应用  

Study on absorption spectrum of chlorosulfophenol N-proteins-triton X-100 system and its analytical application

作　　者：胡秋娈[1] 冯爱青[2] 白小云[1] 

机构地区：[1]洛阳师范学院化学化工学院 [2]洛阳师范学院生命科学系,河南洛阳471022

出　　处：《化学试剂》2011年第1期44-46,共3页Chemical Reagents

基　　金：河南省科技攻关重点项目(102102110154);河南省教育厅自然科学基金资助项目(200510482009)

摘　　要：在pH3.09-4.16的Britton-Robinson缓冲介质中,有机试剂氯磺酚N能与生物大分子蛋白质形成紫红色复合物,λmax=620 nm,比试剂本身红移75 nm,摩尔吸光系数2.01×10^5L·mol^-1·cm^-1,蛋白质在0-140μg·mL^-1范围内遵循比尔定律,Tritton X-100存在反应灵敏度提高。方法操作简便,线性范围宽,基本无干扰,直接用于饲料蛋白的测定,结果满意。Chlorosulfophenol N could bind with proteins to form purplish red complex in a Britton Robinson buffer at pH 3.09～4.16,which presented a maximum absorption at 620 nm with 75 nm of red shift compared to chlorosulfophenol N itself.The molar absorptivity was 2.01×10^5 L·mol^-1·cm^-1.The Beer′s law was obeyed for protein in the range of 0～140 μg/mL（BSA）.The sensitivity increased in the presence of Triton X-100.This method has been used for the determination of protein in the fodder with satisfactory results.

关 键 词：氯磺酚N 吸光光度法 蛋白质 饲料 

分 类 号：O657.32[理学—分析化学]