原发性肺癌D2-40阳性淋巴管密度的检测及其临床意义  

Detection of D2-40-positive lymphatic microvessel density and its clinical significance in lung cancer

作　　者：明宗娟[1] 和平[1] 杨拴盈[1] 刘原[1] 董济民[2] 何积银[1] 

机构地区：[1]西安交通大学医学院第二附属医院呼吸内科,陕西西安710004 [2]陕西省西安市中心医院肿瘤科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2011年第1期23-27,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的检测D2-40在肺癌中的表达,观察微淋巴管在肺癌中的分布及结构特点,探讨淋巴管生成在肺癌发生、发展和转移中的作用及其临床意义。方法采用免疫组化SP法检测65例包含肺癌中心组织、相应癌旁组织以及大致正常组织的肺癌标本中D2-40、CD34的表达,测定微淋巴管密度(LMVD)和微血管密度(MVD),分析二者的相关性及癌旁LMVD与临床病理参量的关系。结果 D2-40标记的LMVD与CD34标记的MVD不相关(P>0.05),肺癌中存在较为活跃的微淋巴管生成,癌旁组织尤为显著,癌旁LMVD与患者的性别和年龄、肿瘤的部位和大小、组织类型以及分化程度无关(P均>0.05),与肿瘤P-TNM分期和淋巴结转移密切相关(P均<0.01)。结论 D2-40是一种较为理想、更为可靠的淋巴管内皮标记物,淋巴管生成对肺癌的发生、发展和转移起重要作用,抗淋巴管生成的治疗将为肺癌的治疗开辟一个新的途径。Objective To detect the expression of D2-40 in lung cancer,observe the distribution and structural characteristics of D2-40-marked lymphatic microvessels,and study the role lym-phangiogenesis played in the oncogenisis,development and metastasis of lung cancer.Methods The expressions of lymphatic endothelial marker D2-40 and vascular endothelial marker CD34 were detected by SP immunohistoc hemistry in 65 samples of lung cancer containing tumor center,paraneoplastic tissue and roughly normal tissue.The relationship between D2-40-LMVD and CD34-MVD was investigated by spearman correlation analysis.The paraneoplastic LMVD were analysed together with clinicopathological parameters.Results Spearman correlation analysis cued that D2-40-LMVD and CD34-MVD were not correlated（P0.05）.Lymphangiogenesis was active in lung cancer;paraneoplastic LMVD was not associated with genders,ages,tumor location and size,tumor histological types and differentiation degrees,but associated with clinical stages as well as metastasis（P0.01）.Conclusion D2-40 is so reliable that can be used as an ideal lymphatic endothelial marker.Lymphangiogenesis may play an important role in the oncogenisis,development and metastasis of cancer.The therapy against lymphangiogenesis may open up a new strategy for tumor treatment.

关 键 词：D2-40 微淋巴管密度(LMVD) 微血管密度(MVD) 肺癌 

分 类 号：R734.2[医药卫生—肿瘤]