嘉陵江出口段饮用源水中的小环藻生物毒性检测  

Determination of Biological Toxicity of Cyclotella in Drinking Source Water at Outlet of Jialing River

作　　者：张智[1] 王敏[1] 郭蔚华[1] 曾晓岚[1] 李楠[1] 袁绍春[1] 

机构地区：[1]重庆大学三峡库区生态环境教育部重点实验室,重庆400045

出　　处：《中国给水排水》2011年第1期49-51,共3页China Water & Wastewater

基　　金：国家"十一五"科技支撑计划项目(2007BAB21B01);国家水体污染控制与治理科技重大专项(2009ZX07315-002);重庆市自然科学基金资助项目(2006BB7136)

摘　　要：嘉陵江是重庆主城区重要的饮用水源,近年来小环藻的暴发可能对嘉陵江源水水质安全构成威胁,为此在筛选硅壳破壁技术的基础上,对嘉陵江出口段饮用源水中小环藻的生物毒性进行了研究。结果表明,与研磨法和反复冻融法相比,超声波法为较理想的破壁方法,其最大破碎率和最佳破碎率分别为99.9%和98.1%,工作参数如下:功率为200 W、时间为20 min、占空比为50%。采用小白鼠异常毒性试验检测小环藻的生物毒性,注射破碎藻液为0.4 mL/只,48 h之内小鼠的进食、活动均正常,生长无异常,表明小环藻无生物毒性。Jialing River is an important source of drinking water for the main districts of Chongqing, and Cyclotella bloom poses a threat to the safety of drinking water. The biological toxicity of Cyclotella in the drinking source water at the outlet of Jialing River was studied based on choosing an optimal wall-breaking technology. The results show that when compared with grinding method and repeated freezing and melting method, the ultrasonic wall-breaking method is more optimal, and the maximum and optimal wall-breaking rates are 99.9% and 98.1% respectively. The optimal conditions of the ultrasonic method are as follows ： ultrasonic power of 200 W, wall-breaking time of 20 rain and duty cycle of 50%. The abnormal toxicity test in white mice was used to determine the biological toxicity of Cyclotella. Within 48 hours after the injection of broken Cyclotella of 0.4 mL, the eating and physical activities of the mice are normal without abnormal growth, indicating that Cyclotella has no biological toxicity.

关 键 词：小环藻 破壁方法 异常毒性 嘉陵江源水 

分 类 号：R123.1[医药卫生—环境卫生学]