一株芘的高效降解菌的选育及其降解性能研究  

Breeding of a high-performance pyrene-degrading strain and its biodegradation performance

作　　者：唐玉斌[1,2] 马姗姗[1] 王晓朝[1] 陈芳艳[1] 

机构地区：[1]江苏科技大学生物与环境工程学院,镇江212018 [2]镇江市海达科技有限公司,镇江212111

出　　处：《环境工程学报》2011年第1期48-54,共7页Chinese Journal of Environmental Engineering

基　　金：江苏省自然科学基金资助项目(BK2009726);江苏省高校自然科学基础研究资助项目(09KJD610002)

摘　　要：旨在为多环芳烃污染环境的生物修复提供微生物资源和科学依据,从某焦化厂曝气池的活性污泥中分离纯化出一株降解芘的优势菌株B-1,该菌株可在以芘为惟一碳源的培养基中生长繁殖,能利用芘的最高浓度为130 mg/L左右。经形态学观察、生理生化实验及l6S rDNA序列分析,初步判断菌株B-1为芽胞杆菌属。投菌量、芘初始浓度、pH和盐度是影响芘降解效率的因素。在投菌量为15%（V/V）、芘初始浓度为20~100 mg/L和温度为30℃的条件下,该菌在pH为4~11、盐度在1%以内可保持对芘良好的降解能力。当芘初始浓度为80 mg/L,投菌量为13%（V/V）,历时10 d,芘的降解效率可达92.4%。A preponderant pyrene-degrading strain B-1 was isolated and purified from activated sludge collected from aeration basin of a coking plant to provide microorganism resource and scientific basis for bioremediation of the environment polluted by polycyclic aromatic hydrocarbons.Strain B-1 could grow and breed in liquid mineral salt medium with pyrene as sole carbon source and resist pyrene with concentration up to about 130 mg/L.Strain B-1 was initially identified as Bacillus by the results of morphological observation,physio-biochemical test and 16S rDNA sequence analysis.The incubation amount,initial pyrene concentration of liquid medium,pH and salinity are the factors to affect pyrene degradation efficiency.Under the conditions of incubation amount of 15%（V/V）,initial pyrene concentration of 20~100 mg/L and temperature of 30 ℃,strain B-1 could preserve high degradation activities of pyrene in the range of the pH 4~11 and the salinity less than 1%.With the initial pyrene concentration of 80 mg/L,incubation amount of 13%（V/V）,pyrene degradation percentage by strain B-1 was 92.4% after 10 days.

关 键 词：芽孢杆菌 16S RDNA 芘 生物降解 

分 类 号：X172[环境科学与工程—环境科学]