上调基因-4在结肠癌中的表达及临床意义  

Expression of up-regulated gene-4 in colon cancer and its clinicopathologic significance

作　　者：崔浩[1] 张超[1] 刘涛[1] 王攀[1] 徐建华[1] 

机构地区：[1]第三军医大学西南医院全军普通外科中心微创胃肠外科中心,重庆400038

出　　处：《第三军医大学学报》2011年第1期33-35,共3页Journal of Third Military Medical University

基　　金：全军医学科研"十一五"计划面上项目(06MB243)~~

摘　　要：目的探讨上调基因-4(up-regulated gene-4,URG-4)在结肠癌及其癌旁组织中的表达及临床意义。方法收集2010年1-6月本院手术切除的82例结肠癌、癌旁组织样本及石蜡切片标本,用RT-PCR、免疫组化检测URG-4基因的转录和表达情况,分析其与临床病理参数的关系。结果结肠癌组织中的URG-4 mRNA阳性率为85.37%(70/82),显著高于癌旁组织中的36.59%(30/82)(P<0.01)。URG-4蛋白在结肠癌中的表达率为70.73%(58/82),癌旁组织为24.39%(20/82),两组比较差异非常显著(P<0.01);肿瘤组织中URG-4的表达与患者的年龄、性别、肿瘤的分化程度、有无远处转移无明显相关性(P>0.05),而与浸润深度及淋巴结转移显著相关(P<0.01)。结论与癌旁组织相比URG-4在结肠癌中高表达,并且URG-4表达强度与肿瘤浸润深度和淋巴结转移呈正相关,提示其可能在结肠癌的发生、发展中起重要作用。Objective To investigate the expression of up-regulated gene-4（URG-4） in colon cancer tissues and adjacent non-cancerous tissues and its clinicopathologic significance.Methods Paraffin-embedded and liquid-nitrogen-preserved specimens of colon cancer tissues and adjacent non-cancerous tissues were prepared from 82 colon cancer patients in our hospital from January to June in 2010.Expression of URG-4 mRNA and protein was detected by reverse transcription PCR（RT-PCR） and immunohistochemical staining,and its correlation with clinicopathologic features was analyzed.Results The positive rate of URG-4 mRNA expression in colon cancer tissues（85.73%,70/82） was significantly higher than that in adjacent non-cancerous tissues（36.59%,30/82）（P0.01）.The expression rate of URG-4 protein in colon cancer tissues（70.73%,58/82） was significantly higher than that in adjacent non-cancerous tissues（24.39%,20/82）（P0.01）.In addition,the expression of URG-4 protein was positively related to tumor infiltration depth and lymph node metastasis（P0.01）,while no correlation was found between the expression of URG-4 protein and age,gender,tumor differentiation and distant metastasis（P0.05）.Conclusion The expression of URG-4 mRNA and protein in colon cancer tissues is significantly higher than that in adjacent non-cancerous tissues.Besides,it is positively correlated with tumor infiltration depth and lymph node metastasis in colon cancer.The results indicate that URG-4 plays an important role in colon cancer development.

关 键 词：上调基因-4 结肠癌 RT-PCR 免疫组化 

分 类 号：R730.23[医药卫生—肿瘤] R730.43[医药卫生—临床医学]