复方奥硝唑-甲磺酸培氟沙星缓释制剂对人牙周膜细胞增殖的影响  

Effects of compound ornidazole-pefloxacin mesylate sustained-release preparation on human periodontal ligament cell proliferation

作　　者：刘宁[1] 刘鲁川[1] 董正谋[1] 刘娜[1] 安建平[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所口腔科,重庆400042

出　　处：《第三军医大学学报》2011年第1期78-81,共4页Journal of Third Military Medical University

基　　金：重庆市科技攻关项目(CSTC2009AC5019)~~

摘　　要：目的观察不同浓度复方奥硝唑-甲磺酸培氟沙星缓释制剂对人牙周膜细胞(human periodontal ligament cells,HPDLC)生长及ki-67表达的影响。方法组织块法培养原代HPDLC,MTT法检测HPDLC在复方奥硝唑-甲磺酸培氟沙星缓释制剂的不同浓度(0、1.25、2.5、5、10、20 g/L)下的光密度值,检测细胞的相对增殖率;采用流式细胞仪检测细胞周期;免疫荧光法检测ki-67的阳性表达率。结果在1.25、2.5 g/L的浓度下对HPDLC的生长和ki-67的表达与对照组比较差异均无统计学意义(P>0.05);而5、10 g/L浓度组的光密度值有所增大,细胞增殖指数提高,ki-67的阳性表达率也有所增加,与对照组比较差异有统计学意义(P<0.05);高浓度药物20 g/L对HPDLC的生长有一定的抑制作用。结论 5、10 g/L的复方奥硝唑-甲磺酸培氟沙星缓释制剂对体外培养的HPDLC的生长增殖有一定促进作用。Objective To study the effects of different concentrations of compound ornidazole-pefloxacin mesylate sustained-release preparation on the proliferation of human periodontal ligament cells（HPDLCs） and the expression of ki-67.Methods Primary HPDLCs were isolated through tissue explant culture technique,and cultured in media containing different concentrations（0,1.25,2.5,5,10 and 20 g/L） of compound ornidazole-pefloxacin mesylate sustained-release preparation.MTT assay was then utilized to measure the OD values of HPDLCs to reflect the relative growth rates.Cell cycles were detected by flow cytometry.The positive expression rates of ki-67 were detected by immunofluorescence assay.Results In the 1.25 and 2.5 g/L groups,HPDLC proliferation and ki-67 expression showed no significant difference from the control group（P0.05）.In the 5 and 10 g/L groups,the OD values,cell proliferation indexes,and ki-67 positive expression rates increased significantly,with statistical differences from the control group（P0.05）.In the 20 g/L group the cell proliferation was inhibited.Conclusion Two concentrations（5 and 10 g/L） of compound ornidazole-pefloxacin mesylate sustained-release preparation can promote the proliferation of HPDLCs in vitro.

关 键 词：牙周炎 牙周膜细胞 增殖 周期 KI-67 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R781.4[医药卫生—基础医学]