亚硝酸钠对红色毛癣菌形态学的影响  

Effects of sodium nitrite on Trichophytosis rubrum

作　　者：佟盼琢[1] 谢红霞[1] 杨云[1] 杨晓亮[1] 冯琴[1] 王冰心[1] 

机构地区：[1]河北医科大学第二医院皮肤性病科,河北石家庄050000

出　　处：《临床皮肤科杂志》2011年第1期13-15,共3页Journal of Clinical Dermatology

摘　　要：目的:观察亚硝酸钠对红色毛癣菌生长的抑制作用。方法:应用美国临床实验室标准化委员会(CLSI)制订的M38-A方案,采用四甲基偶氮唑蓝(MTT)微量稀释法测定亚硝酸钠对红色毛癣菌的体外抑制率。将亚硝酸钠作用前、后的红色毛癣菌制成标本,分别在扫描电子显微镜(扫描电镜)和透射电子显微镜(透射电镜)下观察形态学变化。结果:浓度为160、80、40、20、10、5、2.5、1.25 mmol/L时,亚硝酸钠对红色毛癣菌的抑制率分别为:73.62%±9.7%、71.93%±1.47%、73.53%±1.14%、70.80%±1.50%、68.67%±1.27%、41.76%±1.53%、29.59%±1.90%、16.98%±1.82%。随药物浓度减小,细胞抑制率逐渐降低,经单因素方差分析,差异有统计学意义(P<0.05)。亚硝酸钠作用于红色毛癣菌后,扫描电镜下菌丝表面变粗糙,菌丝萎缩、皱瘪、粗细不一,出现多处破损、断裂和小的破坏性孔洞。透射电镜下细胞变形,呈不规则形;细胞壁粗糙,厚薄不均;细胞质结构模糊,细胞核凝固、破碎。结论:亚硝酸钠可以抑制红色毛癣菌的生长并使之发生形态变化。Objective： To observe the morphological changes of cultured Trichophytosis rubrum treated by sodium nitrite in vitro. Methods： MTT [3-（4, 5---dimethylthia zol-2-yl）-2,5--diphen- yltetrazolium bromide] microdilution method was used to evaluate the inhibition of Trichophyton rubrum by sodium nitrite in vitro, according to the recommendations of the CLSI（M38-A）. The uhrastructural morphology of the fungal strains before and after the sodium nitrite treatment was observed under the scanning electron microscope and transmission electron microscope. Results： As the concentrations of sodium nitrite as 160 mmol/L, 80 mmol/L, 40 mmol/L, 20 mmol/L, 10 mmol/L, 5 mmol/L, 2.5 mmol/L, or 1.25 mmol/L, the inhibition rates of Trichophyton rubrum were 73.62% ± 9.7%, 71.93% ± 1.47%, 73.53% ±1.14%, 70.80% ± 1.50%, 68.67% ± 1.27%, 41.76%± 1.53%, 29.59% ± 1.90%, or 16.98% ± 1.82%, respectively. With the concentrations of sodium nitrite decreased, a statistically gradual reduction in the inhibition rate of Trichophyton rubrum was seen （P 〈0.05）. The scanning electron microscopy showed the hyphae were damaged and broken, the surface of the hyphae became rough and shrunken, with many destructive fissures and small holes on it. The transmission electron microscopy showed the hyphae became deformed and irregular, the cell wall was roughly and loosely, the cytoplasm texture obscured and cellular nucleus was of syneresis and quassation. Conclusions： The growth of Trichophytosis rubrum could be inhibited by sodium nitrite in vitro and the morphology of Trichophytosis rubrum could be changed.

关 键 词：亚硝酸钠 红色毛癣菌 扫描电子显微镜 透射电子显微镜 

分 类 号：R756.6[医药卫生—皮肤病学与性病学]