肝细胞黏附分子诱导肾癌786-0细胞系凋亡  

HEPACAM induces apoptosis in renal cell carcinoma 786-0 cell line

作　　者：张巧琳[1] 罗春丽[1] 颜令[1] 吴小候[2] 

机构地区：[1]重庆医科大学医学检验系临床检验诊断学教育部重点实验室重庆市重点实验室,重庆400016 [2]重庆医科大学泌尿外科,重庆400016

出　　处：《基础医学与临床》2011年第1期73-77,共5页Basic and Clinical Medicine

摘　　要：目的探讨肝细胞黏附分子(HEPACAM)基因转染肾癌细胞对其凋亡的影响。方法将携带HEPACAM基因的重组质粒转染786-0细胞,RT-PCR鉴定HEPACAM基因在786-0中的表达;Hoechst染色结合荧光显微镜观察细胞形态改变;FCM检测细胞周期及细胞凋亡情况;Western印迹法检测BAX、BCL-2、Cyclin D1蛋白表达变化。结果上调HEPACAM基因表达可诱导786-0细胞凋亡,阻止细胞周期于G0/G1期,并可上调BAX及下调BCL-2和Cyclin D1蛋白水平表达。结论外源导入HEPACAM基因可诱导肾癌细胞凋亡并阻止细胞周期于G0/G1期。Objective To investigate the effect of transfection of hepatocyte cell adhesion molecule（HEPACAM） on apoptosis of renal cell carcinoma cells.Methods The vector containing HEPACAM gene was transfeceted into 786-0 cells,the expression of HEPACAM gene in 786-0 cells was confirmed by RT-PCR;The morphological change of nucleus was shown by Hoechst staining;The proliferation and apoptosis of 786-0 cells were analyzed by FCM;The expression of BAX,BCL-2,Cyclin D1 was measured by Western blot.Results Up-regulated expression of HEPACAM gene in 786-0 cells can arrest cell cycle at G0/G1 phase and induce cell apoptosis,the expression of BAX increased and the expression of BCL-2 and Cyclin D1 decreased.Conclusion Ectogenic transfected HEPACAM gene may induce cell apoptosis and arrest cell cycle at G0/G1 phase in renal cell carcinoma cells.

关 键 词：肾癌 肝细胞黏附分子 细胞周期 凋亡 

分 类 号：R737.11[医药卫生—肿瘤]