竹小叶病植原体的分子鉴定  

Identification of phytoplasma associated with bamboo little leaf disease

作　　者：张春平[1] 武占敏[1] 李正男[1] 张珏[2] 宋加贵[1] 杨洋[2] 吴云锋[1] 

机构地区：[1]西北农林科技大学植保学院与陕西省农业分子生物学重点实验室,植保资源与病虫害治理教育部重点实验室,杨凌712100 [2]西北农林科技大学生命科学学院,杨凌712100

出　　处：《植物病理学报》2011年第1期31-36,共6页Acta Phytopathologica Sinica

基　　金：国家自然科学基金项目(30871625);高等学校学科创新引智计划项目(B07049)

摘　　要：2008年在杨凌采集到具有典型植原体侵染症状的菲白竹,应用植原体16S rRNA基因的通用引物对R16mF2/R16mR1和R16F2n/R16R2对其进行检测,巢式PCR得到约1.2 kb的特异性片段。对扩增片段进行测序并进行系统进化树分析,结果表明,该病原属于翠菊黄化组(Candidatus Phytoplasma asteris),与该组成员同源性均在98%以上。随后用16SrⅠ组和Ⅴ组特异引物对R16(Ⅰ)F1/R16(Ⅰ)R1和R16(Ⅴ)F1/R16(Ⅴ)R1也证明其属于翠菊黄化组,RFLP分析表明该植原体属于16SrⅠ-B亚组。植原体侵染菲白竹在中国属首次报道。Typical symptoms of phytoplasma infection were observed in Sasa fortunei （van Houtte）Fiori during a disease survey in Yangling. Using 16S rRNA universal phytoplasma primer pairs R16mF2/R16mRland R16F2n/R16R2, 1.2 kb specific fragment was obtained from bamboo with symptoms of little leaf and stunt. The fragment was sequenced and subjected to RFLP and phylogenetic analyses. The strain obtained from bamboo was belonged to ＂Candidatus Phytoplasma asteris＂ and shared identities of more than 98% with other members in this group. Group specific primer pairs R16（ I ） F1/R16 （ I ） R1 and R16 （ V ） F1/R16 （ V ） R1 confirmed that the strain was a member of group ＂Candidatus Phytoplasma asteris＂. And it also revealed that no complex infection was existed, RFLP analysis showed that the phytoplasma was a member of 16Sr I -B subgroup. This is the first report of phytoplasma infection in S. fortunei （ van Houtte） Fiori.

关 键 词：竹小叶病 植原体 16S RRNA基因 RFLP 系统进化分析 

分 类 号：S436.65[农业科学—农业昆虫与害虫防治]