贵州地区牛源金黄色葡萄球菌分离株16S rRNA分子鉴定及RAPD分型研究  

Research of Identification by 16S rRNA and RAPD Typing on Staphylococcus aureus Isolated from Dairy Cattle in Guizhou

作　　者：邓海平[1] 蒲万霞[1] 梁剑平[1] 倪春霞[1] 孟晓琴[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,中国农业科学院新兽药重点开放实验室,甘肃省新兽药重点实验室,甘肃兰州730050

出　　处：《中国畜牧兽医》2011年第1期112-115,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家科技支持计划(2006BAD04A05)

摘　　要：利用16S rRNA保守序列对引起贵州地区奶牛乳房炎的金黄色葡萄球菌进行分离鉴定,建立随机多态性扩增体系对金黄色葡萄球菌分离株进行基因分型研究。结果表明,69份奶样中共分离出金黄色葡萄球菌37株,DNA随机多态性扩增（random amplified polymorphic DNA,RAPD）结果显示这37株金黄色葡萄球菌扩增产物在1~7条带之间,产物大小为400~4500 bp。菌株可分为7个基因型,其中Ⅰ型7株（18.9%）、Ⅱ型2株（5.4%）、Ⅲ型2株（5.4%）、Ⅳ型1株（2.7%）、Ⅴ型19株（51.4%）、Ⅵ型1株（2.7%）、Ⅶ型5株（13.5%）,Ⅴ型为该地区的流行优势菌群。地理和气候环境对病原菌流行传播的影响是病原菌基因型分布差异的重要原因。The 16S rRNA conserved sequence was used for isolation and identification of Staphylococcus aureus（S.aureus） strains causing bovine mastitis in Guizhou,and system of randomly amplified polymorphic DNA（RAPD） was established for research on genetic polymorphism of the S.aureus strains.The results showed that 37 S.aureus strains were isolated and identified from 69 mastitis milk samples.The 37 S.aureus strains amplified out 1 to 7 electrophoresis strips with sizes ranging from 400 to 4500 bp by RAPD.The strain were separated to 7 genotypes according to the fingerprint,7 strain were type Ⅰ（18.9%）,2 strains were type Ⅱ（5.4%）,2 strains were type Ⅲ（5.4%）,1 strains was type Ⅳ（2.7%）,19 strains were type Ⅴ（51.4%）,1 strain was type Ⅵ（2.7%） and 5 strains were typeⅦ（13.5%）.The preponderant S.aureus strains in this area was typeⅤ.Effects of geography and climate on transmission of pathogenic bacteria was the mainly reason of discrepancy on proportion of different genotype strains.

关 键 词：奶牛乳房炎 金黄色葡萄球菌 16S RRNA RAPD 基因型 

分 类 号：S855.1[农业科学—临床兽医学]