一氧化氮及一氧化氮合酶抑制剂在大鼠局灶性脑缺血时作用研究  

The effects of nitric oxide and nitric oxide synthase inhibitor after focal cerebral ischemic in rats

作　　者：曹非[1] 孙圣刚[1] 童萼塘[1] 李红莲 魏瑛[3] 

机构地区：[1]同济医科大学附属协和医院神经内科,430022 [2]同济医科大学组织胚胎教研室 [3]同济医科大学脑研究所

出　　处：《卒中与神经疾病》1999年第3期154-157,共4页Stroke and Nervous Diseases

摘　　要：目的 :观察一氧化氮及一氧化氮合酶抑制剂在大鼠局灶性脑缺血时的作用。方法 :用直接电凝大鼠大脑中动脉制成脑缺血模型 ,选择脑缺血 30 min、6 0 min、1 2 0 min、1 80 min为研究时点 ,观察各时点用选择性、非选择性一氧化氮合酶抑制剂 ( AG、L-NAME)亚硝酸盐含量测定、缺血脑组织坏死体积测定及损伤海马 CA1 区电镜观察。结果 :缺血组、用药组从 30分钟到 6 0分钟亚硝酸盐含量呈下降趋势 ,而从 6 0分钟至 1 80分钟又至上升趋势 ,病理及电镜显示 30分钟时用药组损伤重于缺血组 ,而 6 0分钟至 1 80分钟相反。结论 :NO在局灶缺血性脑损伤中呈现双重作用 ,一氧化氮合酶抑制剂对脑缺血后期治疗有效。Objective:To observe the function of nitric oxide and nitric oxide synthase inhibitor after focal cerebral ischemia in rats.Methods:We measured brain nitrites,the infart volume and investigate the CAI region of the hippocampus at 30,60,120,180 min after the middle cerebral artery were blocked continuously in rats.Results:Brain nitrite were decreased from 30 min to 60 min after occlusion and increased from 60 min to 180 min.The pathologic and ultramicroscopic examination in postischemic brain at 30 min showed that the injury of control group was more serious than that of ischemic group.The results from 60 min to 180 min was opposite.Conclusions:These results suggest that nitric oxide play twofold roles in focal cerebral ischemia in rats.Nitric oxide synthase inhibitor is effective in later period of cerebral ischemia.

关 键 词：一氧化氮 一氧化氮合酶 抑制剂 局灶性 脑缺血 

分 类 号：R743.310.5[医药卫生—神经病学与精神病学]