^(99m)Tc-HL91肿瘤乏氧显像及肿瘤坏死对显像影响的实验研究  

Experimental Research on 99m Tc HL91 Tumor Hypoxic Image and the Influence of Tumor Necrosis on the Image

作　　者：姚稚明[1] 屈婉莹[2] 周颖 朱明 朱霖[2] 

机构地区：[1]北京医院核医学科,100370 [2]国家标准物质研究中心

出　　处：《中国医学影像技术》1999年第9期736-738,共3页Chinese Journal of Medical Imaging Technology

基　　金：国家攀登计划B-科研基金

摘　　要：目的　研究乏氧显像剂９９ｍＴｃＨＬ９１ 作为肿瘤阳性显像剂，以及肿瘤坏死对其显像的影响。方法　从人乳腺癌荷瘤裸鼠尾静脉注射９９ｍＴｃＨＬ９１ ７４ ＭＢｑ，采集荷瘤裸鼠图像，处死后测量各脏器、组织和肿瘤的重量和放射性，肿瘤切片，进行放射性自显影和显像。结果　肿瘤显影清晰，注射后６ 小时显像上，肿瘤和头部、胸部的放射性比值分别为２．４４ ±０ ．５３ 、２ ．１３ ±０ ．６４ 。非坏死肿瘤和坏死肿瘤的放射性比值为３７７ ±０ ．３５ 。肿瘤切片的放射性自显影和显像上可见坏死部分放射性低于非坏死部分。肿瘤和腹部、肝脏和肠的放射性比值低。结论　人乳腺癌荷瘤裸鼠的瘤体在９９ｍＴｃＨＬ９１ 显像上为清晰的阳性病灶；坏死肿瘤组织的放射性摄取低于非坏死肿瘤；９９ｍＴｃＨＬ９１在腹部脏器滞留量高，影响其在腹部肿瘤诊断中的应用。Objective To evaluate the hypoxia avid agent 99m Tc HL91 as the tracer of tumor “hot spot image” and the influence of tumor necrosis on the image.Methods Six nude mice bearing human breast cancer MCF 7 were studied. ① Gamma camera imaging:Injection of 37 MBq(1 mCi) of 99m Tc HL91 was followed by static planar imaging.② Postmortem analysis:Mice were sacrificed after injection of 99m Tc HL91 6 hour, the organs and tumor were removed, weighted, measured with radioactivity. ③ Autoradiograph and image of tumor.Results ① Gamma camera imaging:The image of tumor was identified after injection of 99m Tc HL91 1 hour. Images demonstrated increased 99m Tc HL91 uptake in the tumor after injection 1 to 6 hours, gradually ( P <0.05～0.001). ② Postmortem analysis:The radioactivity ratios of tumor to brain, muscle, blood, heart and lung were higher than 4.9 after injection 6 hour. The radioactivity of non necrotic tumor was 3.77 times of that of necrotic tumor. However, the radioactivity ratios between tumor to liver, intestine and stomach were low. ③ The autoradiograph and image of tumor showed obviously higher radioactivity in the region of solid tumor than that in the necrotic region of tumor.Conclusion 99m Tc HL91 positively identifies regional tumor in nude mice bearing breast cancer MCF 7 using gamma camera. Necrotic tumor has significantly lower 99m Tc HL91 retention than non necrotic tumor does. The low radioactivity of tumor to organs in abdomen is the limitation of application of 99m Tc HL91 in detecting tumor in abdomen. [

关 键 词：乏氧显像 锝99m HL91 肿瘤坏死 

分 类 号：R730.44[医药卫生—肿瘤]