山西不同生态型大豆种质资源蛋白亚基的变异  

Variation of protein subunits of soybean germplasms of different eco-types in Shanxi

作　　者：王燕平[1] 李贵全[1] 郭数进[1] 王鹏[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801

出　　处：《生态学报》2011年第1期203-211,共9页Acta Ecologica Sinica

基　　金：国家科技支撑计划资助项目(2006BAD01A04);山西省科技攻关计划资助项目(20080311007-1)

摘　　要：选用山西57份不同生态型大豆种质资源为材料,利用SDS-PAGE梯度电泳技术分离11S球蛋白和7S伴球蛋白各主要亚基,通过Quantity One 4.52软件得出11S和7S及其亚基的相对含量。结果表明,不同生态型大豆种质资源间同一亚基相对含量存在较大变异,其中变异系数最大的为β亚基,变异幅度为7.32%—21.71%,变异系数为17.46%。11S/7S比值平均值为1.78±0.33,变异幅度为1.46%—3.45%,变异系数为18.55%,差异较大。11S、7S含量与蛋白质和脂肪含量没有相关性。可以看出大豆蛋白亚基相对含量随品种和产地变化存在明显的变异。同时发现4份自然变异的特异大豆种质,为专用型优质大豆品种的选育及大豆食品加工原料的选择提供重要的参考种质。Soybean protein is an important source of edible protein.The components of subunits of soybean protein not only directly affect the function of soybean,but also affect the nutritional characteristics of soybean,thus affecting its application value.There were many reports about the soybean germplasms with 11S glycinin and 7S conglycinin and the relative content of their subunits,however,there is no research report on the components and contents of protein subunits of soybean germplasms in Shanxi ecotypes.In this study,57 soybean germplasms of different eco-types from Shanxi Province were tested.pH gradients were set to analyze the total proteins of the same soybean germplasm（51×57） precipitated in different pH values by SDS-PAGE electrophoresis,determining the best pH value（pH=4.6） precipitating storage proteins.Based on the analysis above,various main subunits of 11S glycinin and 7S conglycinin were separated and relative contents of 11S,7S and their subunits were acquired by Quantity One 4.52.The results showed that among the components of 7S,the subunit β possessed the highest average relative content,which was 15.97%;among the components of 11S,the subunit BS possessed the highest average relative content,which was 44.76%.The relative contents of the same subunits of different eco-types soybean germplasms showed wide variation.The subunit β had the highest variation coefficient and its variant range was from 7.32% to 21.71%,its variation coefficient amounted to 17.46%.The subunit BS had the lowest variation coefficient and its variant range was from 36.65% to 51.86%,its variation coefficient was only 5.65%.The average value of the 11S/7S ratio of soybean ecotopes in Shanxi was 1.78,standard deviation was 0.33,the variant range was from 1.46 to 3.45 and the variation coefficient was 18.55%.The 11S/7S ratios were mostly distributed between 1.50 and 1.99,there were 43 germplasms in the range.There were 5 germplasms whose 11S/7S ratios were lower than 1.50 and there were 4 germplasms whose 11S/7S ratio

关 键 词：山西 大豆 种质资源 蛋白亚基 变异 

分 类 号：S565.1[农业科学—作物学]