不同来源苦荞中芦丁和槲皮素的含量测定  

Determination of the Contents of Rutin and Quercetin in Buckwheat from Different Sources

作　　者：宾婕[1] 刘洁[1] 陈克嶙[2] 周劲 郭荣富[2] 郭亚东[1] 

机构地区：[1]昆明医学院药学院,云南昆明650500 [2]云南农业大学动物科技学院,云南昆明650201 [3]云南侨通包装印刷有限公司,云南昆明657000

出　　处：《现代食品科技》2011年第1期117-119,共3页Modern Food Science and Technology

基　　金：云南侨通包装印刷有限公司资助项目(kx140564)

摘　　要：建立了一种苦荞中芦丁和槲皮素含量的测定方法。以超声法进行提取,并分别以芦丁和槲皮素为标品,采用岛津ODS柱(4.6 mm×150 mm,5μm),以乙腈-水(含2%的冰醋酸)为流动相,梯度洗脱,流速:1 mL·min-1,检测波长:360 nm。芦丁进样量在0.023～1.150μg范围内(r=0.9996),槲皮素进样量在0.02～0.60μg范围内(r=0.9999)线性关系良好。平均加样回收率芦丁为98.5%,槲皮素为95.8%。该方法简便、快速、准确,可测定苦荞中芦丁和槲皮素的含量。A new method for determination of rutin and quercetin contents in buckwheat was developed in this paper.The extracts from buckwheat by Ultrasonic extraction was analyzed on a Shimadzu ODS column（4.6 mm×150 mm,5 μm）with a mobile phase of acetonitrile-water（containing 2% glacial acetic acid） for gradient elution at 360nm.And the flow rate was 1.0 mL/min.The linear ranges of rutin and quercetin concentrations were found within 0.023～1.150 μg（r=0.9996） and 0.02～0.60 μg（r=0.9999）,respectively.The average recoveryies of rutin and quercetin were 98.5% and 95.8%,respectively.The method was simple,accurate and showed good reproducibility,which was suitable for the determination of rutin and quercetin contents in buckwheat.

关 键 词：苦荞 芦丁 槲皮素 高效液相色谱 含量测定 

分 类 号：R284[医药卫生—中药学]