马尾松根部蛋白双向电泳分离体系的构建  

Establishment of two-dimensional gel electrophoresis system for analyzing the root protein of Pinus massoniana

作　　者：徐超[1] 吴小芹[1] 林司曦[1] 张红岩[1] 

机构地区：[1]南京林业大学,江苏省有害生物入侵预防与控制重点实验室,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2011年第1期15-18,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家自然科学基金项目(30571471);“十一五”国家科技支撑计划项目(2006BAD08A1002)

摘　　要：为了在蛋白质水平探讨外生菌根提高马尾松抗旱能力的机制,笔者通过对菌根化和非菌根化马尾松植株根部蛋白提取和SDS-聚丙烯酰胺凝胶染色条件进行研究,建立了一套较为适合马尾松根部蛋白双向电泳分离的试验体系。结果表明:在每IPG胶条300μg上样量条件下,采用酚抽法辅以适度超声波破碎进行马尾松根部蛋白提取、敏化和银染后水洗时间均为5min×3次的快速银染程序进行染色,可以获得较为理想的马尾松根部蛋白双向电泳图谱,该方法可为其他松属树种的蛋白质组学等研究提供参考。Plant vital activities could be revealed on protein level.Obtaining good two-dimensional gel electrophoresis（2-DE） profile is the precondition for experimental procedures such as mass spectrum analysis,and so on.In order to explore the mechanism of mycorrhiza underlying drought resistance of Pinus massoniana on protein level,a set of optimized experiment system of 2-DE for analyzing root protein extracted from P.massoniana has been established.The ideal 2-DE profile could be obtained when protein was extracted by phenol extraction and shattered the cells with ultrasonic crasher,and then the gels were stained by fast silver stain and washed three times for 5 min after sensitizing and dying.The suitable concentration of protein was 300 μg per IPG gel.It also provided reference for the proteomic studies on other pine species.

关 键 词：菌根 根部蛋白 双向电泳 马尾松 

分 类 号：S718.4[农业科学—林学]