NSCLC组织中MGMT过甲基化状况与临床病理特征的关系探讨  

Investigation on the relationgship between the promoter hypermethylation of MGMT and clinicopathologic feature in NSCLC tissues

作　　者：亢春彦[1] 陶志敏[2] 肖红[1] 张秀芝[1] 薛玉仙[1] 

机构地区：[1]河南职工医学院病理学教研室,河南郑州451191 [2]河南大学护理学院

出　　处：《中国实验诊断学》2011年第1期81-82,共2页Chinese Journal of Laboratory Diagnosis

基　　金：河南省卫生厅医学科技攻关资助项目(200903116)

摘　　要：目的探讨非小细胞肺癌(NSCLC)组织中DNA修复酶O6-甲基鸟嘌呤-DNA甲基转移酶(MGMT)过甲基化状况与临床病理特征的关系。方法以2006年3月到2009年7月在河南省胸科医院胸外科手术治疗的77例NSCLC患者作为研究对象,另外选取20例肺良性病变患者作为对照组,采用甲基化特异性PCR(MSP)检测两组病例肺部病变组织中MGMT的甲基化状况。结果研究组有26例出现MGMT基因甲基化,发生率为33.8%,对照组无1例出现MGMT基因甲基化,组间比较差异有统计学意义(P<0.05);NSCLC中MGMT过甲基化状态与有吸烟史、有淋巴结转移、TNM分期为Ⅲ期和分化程度为低分化有关,与患者年龄、性别和组织学类型无关。结论 NSCLC组织中的MGMT过甲基化状况可能与肺癌的发生、发展相关。Objective To investigate the relationgship between the promoter hypermethylation of MGMT gene and clinicopathologic feature in NSCLC tissues.Methods Took 77 cases of NSCLC who were treated in Henan Chest Hospital from March 2006 to July 2009 as research subjects,the other 20 cases with benign lung disease as the control group,the methylation specific PCR（MSP） were used to test the methylation status of MGMT in two groups.Results 26 cases of study group had MGMT gene methylation,the incidence rate was 33.8%,while no case of control group occurred MGMT gene methylation,the difference between groups was statistically significant（P0.05）;The promoter hypermethylation of MGMT was correlated to the history of smoking,lymph node metastasis,TNM stage was Ⅲ and low degree of tumor differation（P0.05）,but not correlated to patient age,gender and histological type（P0.05）.Conclusion The promoter hypermethylation of MGMT gene may take part in lung carcinoma evolvement.

关 键 词：非小细胞肺癌 O6-甲基鸟嘌呤-DNA甲基转移酶 甲基化 病理特征 

分 类 号：R734.2[医药卫生—肿瘤]