趋化因子受体7在膀胱癌组织中的表达及其临床意义  

Expression of CXCR7 protein in human bladder cancer and its clinical significance

作　　者：侯恺林[1] 张连华[1] 薄隽杰[1] 郝曼罡[2] 杨国良[1] 蒋海锋[1] 刘东明[1] 王建华[2] 黄翼然[1] 

机构地区：[1]上海交通大学医学院附属仁济医院泌尿外科,200001 [2]上海交通大学医学院生物化学与分子细胞学系

出　　处：《中华泌尿外科杂志》2011年第1期42-46,共5页Chinese Journal of Urology

基　　金：基金项目：上海市科委自然基金（09ZR14180000）;上海市教科委肿瘤学重点学科（J50208）

摘　　要：目的探讨趋化因子受体7（CXCR7）蛋白在膀胱癌中的表达情况，并分析其与临床生物学行为及复发的关系。方法选择148例膀胱移行细胞癌标本，30例正常膀胱组织标本：肿瘤标本中非肌层浸润性癌104例，肌层浸润性癌44例；G147例、G2 73例、G3 28例；单发肿瘤89例，多发肿瘤59例；非肌层浸润性癌中未复发40例，复发64例。采用LSAB免疫组织化学方法，检测膀胱癌和正常组织中CXCR7蛋白的表达，并结合临床资料进行分析。结果正常组织中CXCR7蛋白的表达水平为10．0％（3／30），肿瘤组织为85．1％（126／148）；单发及多发肿瘤CXCR7蛋白高表达率分别为49．4％（44／89）和71．2％（42／59），G1、G2、G3膀胱癌CXCR7蛋白高表达阳性率分别为34．0％（16／47）、65．8％（48／73）、78．6％（22／28），差异均有统计学意义（P〈0．01）。非肌层浸润性肿瘤和肌层浸润性肿瘤中CXCR7蛋白高表达阳性率为51．9％和72．7％，组间差异有统计学意义（P〈0．05）。非肌层浸润性膀胱癌中，复发组与未复发组中CXCR7蛋白高表达阳性率为64．1％和32．5％，差异有统计学意义（P〈0．01）。KaplanMerier曲线显示，CXCR7蛋白高表达组患者和CXCR7蛋白低表达组患者术后无复发生存率比较，差异有统计学意义（P〈0．01）。结论CXCR7蛋白高表达与膀胱移行细胞癌的恶性程度及预后相关，提示CXCR7与膀胱癌的发生发展存在相关性。Objective To explore the expression of CXCR7 in bladder cancer and analyze its clinical significance and relationship with bladder cancer recurrence. Methods The expressions of CXCR7 protein in 148 specimens of bladder cancer and 30 specimens of normal bladder tissues were detected by immunohistoehemical staining and its clinical significance was then analyzed. Results The expression of CXCR7 protein was higher in bladder cancers than in the adjacent normal tissues （P〈0.01）. CXCR7 protein expression rates were 49.4% and 71.2% in mutifoeal tumors and unifoeal tumors, while 34.0%, 65.8% and 78.6% in G1 , G2, and G3 tumors, respectively （P〈0. 01）. Expression of CXCR7 protein was higher in muscle invasive bladder cancers than in non-muscle invasive bladder cancers （72.7% versus 51.9% ,P〈0. 05）. In patients followed up for 2--95 months, CXCR7 protein expression was significantly higher in patients with recurrence than with non-recurrence （64.1 % versus 32.5%, P〈0.01）. Kaplan-Meier analysis and the log-rank test showed that the recurrence-free survival was significantly different between the group of lower CXCR7 expression group and the higher expression group （P〈0.01）. Conclusions The expression of CXCR7 protein is high in bladder cancer and the analysis of CXCR7 protein expression is potentially valuable in prognostic evaluation of bladder cancers, CXCR7 may play a role in the development of bladder urothelial cellcancer.

关 键 词：膀胱肿瘤 CXCR7 免疫组织化学 复发 

分 类 号：R737.14[医药卫生—肿瘤]