电导抑制离子色谱法测定八角中莽草酸含量  

Determination of Shikimic Acid in Chinese Star Anise Fruit by Ion Chromatography with Suppressed Conductivity Detection

作　　者：朱朝娟[1] 周光明[1] 余娜[1] 

机构地区：[1]西南大学化学化工学院,发光与实时分析教育部重点实验室,重庆400715

出　　处：《食品科学》2011年第2期154-156,共3页Food Science

基　　金：国家科技重大专项(2008ZX07315);重庆市自然科学基金项目(CSTC2007BB5370)

摘　　要：建立电导抑制离子色谱法测定八角中莽草酸含量的方法。用MetrohmAsupp4(4mm×250mm)型阴离子分析柱,以1.8mmol/LNa2CO3+1.7mmol/LNaHCO3作淋洗液,淋洗液流速为0.8mL/min,分析时间15min。在优化色谱条件下,莽草酸在1～100mg/L范围内,质量浓度与峰面积有良好线性关系(r=0.9993),检出限为0.02～0.8mg/L。连续6次进样,峰面积的相对标准偏差为1.04%,保留时间的相对标准偏差为0.47%,样品加标回收率为97.3%～98.9%。该方法快速,样品前处理简单,结果满意。A method for the determination of shikimic acid in Chinese star anise（Illicium verum Hook.f.）fruit by ion chromatography with suppressed conductivity detection was developed.The chromatographic separation was conducted on Metrohm A supp 4（4 mm×250 mm）anion exchange column,using 1.8 mmol/L Na2CO3＋1.7 mmol/L NaHCO3 as a mobile phase at a flow rate of 0.8 mL/min.The total analysis time was 15 min.The linear range was between 1 mg/L and 100 mg/L and the correlation coefficient was 0.9993.A detection limit of 0.02-0.8 mg/L was achieved under optimized separation conditions.The RSD of peak area was 1.04%（n=6）,and the RSD of retention time was 0.47%.The average spike recoveries were between 97.3%and 98.9%.This method is fast,simple and reliable.

关 键 词：莽草酸 离子色谱 电导抑制检测 

分 类 号：O652.2[理学—分析化学]