高效液相色谱法同时测定动物组织中16种苯并咪唑类药物残留  

Simultaneous HPLC Determination of 16 Benzimidazoles Residues in Animal Tissue

作　　者：林海丹[1] 林峰[1] 张美金[1] 谢守新[1] 吴映璇[1] 邵琳智[1] 姚仰勋[1] 

机构地区：[1]广东出入境检验检疫局检验检疫技术中心,广东广州510623

出　　处：《食品科学》2011年第2期231-236,共6页Food Science

摘　　要：建立同时测定动物组织中16种苯并咪唑类药物及其代谢物多残留的高效液相色谱分析方法。试样经乙酸乙酯、50g/100mL氢氧化钾溶液、1g/100mLBHT溶液(20+0.15+1,V/V)提取后,正己烷脱脂,MCX固相萃取柱净化,用高效液相色谱C18柱分离,乙腈-0.025mol/L乙酸铵作为流动相,流速为1.0mL/min,梯度洗脱,紫外检测波长为292nm,外标法峰面积定量。16种苯并咪唑类药物在0.025～1.0mg/L质量浓度范围内线性良好,相关系数r均大于0.99,动物组织样品在10、50、100μg/kg添加水平的添加回收率在81.6%～110%之间,相对标准偏差为1.8%～13.1%(n=10),方法的定量限为10μg/kg。方法灵敏度高、重现性好,适用于动物组织中苯并咪唑类药物多残留的检测。A high performance liquid chromatography（HPLC）method was developed for the simultaneous determination of 16 benzimidazoles residues in animal tissue.Animal tissue samples were extracted with ethyl acetate-50%potassium hydrox-ide-1%BHT（20：0.15：1,V/V）,followed by treatment with n-hexane for defatting and further clean-up on MCX solid phase（SPE） cartridge.The chromatographic separation was performed on a C18 column with a mobile phase consisting of acetonitrile and 0.025 mol/L ammonium acetate solution at a flow rate of 1.0 mL/min by gradient elution.Detection wavelength was set at 292 nm,and the quantitation method used was external standard method.The calibration curves of 16 benzimidazoles exhibited good linearity over a concentration range from 0.025 to 1.0 mg/L,with correlation coefficients above 0.99.The average recoveries for 16 benzimidazoles in meat and liver tissues from three different animal species ranged from 81.6%to 110%at spike levels of 10,50μg/mL and 100μg/mL,and the relative standard deviations were between 1.8%and 13.1%（n=10）.The limit of quantification was 10μg/kg.This method is sensitive,reproducible and suitable for the determination of benzimidazoles in animal tissue.

关 键 词：高效液相色谱 动物组织 苯并咪唑类药物 

分 类 号：O657.72[理学—分析化学]