超高效液相色谱串联质谱快速测定饮用水中9种N-亚硝胺的新方法  

Rapid and sensitive method for simultaneous determination of nine N-nitrosamines in drinking water by UPLC-MS/MS

作　　者：罗茜[1] 王东红[1] 王炳一[1] 张子秋[1] 王子健[1] 

机构地区：[1]环境水质学国家重点实验室,中国科学院生态环境研究中心,北京100085

出　　处：《中国科学：化学》2011年第1期82-90,共9页SCIENTIA SINICA Chimica

基　　金：中国科学院重大项目(KZCX1-YW-06-02);自然科学基金面上项目(50878205);国家高技术研究发展计划重点项目(2008AA062501)资助

摘　　要：N-亚硝胺是潜在的人类致癌物,是近年来关注的一类饮用水消毒副产物,同时也是环境分析研究的热点.本文建立了超高效液相色谱(UPLC)串联质谱快速测定饮用水中9种N-亚硝胺的新方法,讨论了色谱柱和流动相对分离9种N-亚硝胺的影响,优化了多级反应质谱(MRM,MS/MS)条件.二甲基亚硝胺-d6为贮存和回收率内标,亚硝基二丙胺-d14为定量内标,UPLC-MS/MS能够直接快速检测饮用水中热稳定和热不稳定的9种N-亚硝胺.UPLC-MS/MS直接进样方法对二甲基亚硝胺(NDMA)定性和定量检测限分别为0.1和1.0ng/L,亚硝基二丙胺为2.5和10ng/L,其他7种N-亚硝胺的定性和定量检测限均为0.5和2.0ng/L,本方法中N-亚硝胺的检测限低于美国环保局规定水中的最大容许浓度.应用此方法测定了某使用氯胺消毒自来水厂不同工艺段(进水、沉淀和出水)水中N-亚硝胺的浓度和分布.数据表明在自来水厂处理后出厂水中3种具有高致癌风险亚硝胺化合物中NDMA的浓度为3.5ng/L,NMEA和NDEA均未检出,因而目前该城市饮用水中N-亚硝胺的浓度对城市居民的健康没有风险.N-Nitrosomines, a group of probable human carcinogens, has been detected as a disinfection by-products （DBPs） in drinking water. To comprehensively investigate the occurrence of possible nitrosamines in drinking water, an ultra-performance liquid chromatography-tandem mass spectrometry technique was developed to detect both thermally stable and unstable total nine nitrosamines. With optimized liquid chromatography column, mobile phases, and triple quadrupole conditions, UPLC-MS/MS enabled the determination of ng/L levels of nine nitrosamines. Isotope-labeled N-nitrosodimethylamine-d6 （NDMA-d6） was used as the surrogate standard for determining recovery, and N-nitrosodi-n-propylarnine-dl4 （NDPA-dl4） was used as the internal standard for quantification. The limits of detection （LOD） and limits of quantification （LOQ） for NDMA were estimated to be 0.1 and 1 ng/L, NDPA were 2.5 and 10 ng/L, and other seven nitrosamines were 0.5 and 2.0 ng/L, respectively, which were less than maximum permissible concentration by US EPA. The occurrence and distribution of N-nitrosamines in different treatment processes （raw water, deposition and finished water） in drinking water treatment plants with chloramines disinfectant were determined using UPLC-MS/MS with direct injection method. The three nitrosamines with carcinogen risk, NDMA is found in finished water with concentration 3.5 ng/L, NMEA and NDEA were lower than LOQ. This indicated N-nitrosamines in drinking water that could not lead to the health risk for urban residents.

关 键 词：超高效液相色谱串联质谱 饮用水 N-亚硝胺 二甲基亚硝胺 健康风险 

分 类 号：R123.1[医药卫生—环境卫生学]